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PCNA Monoclonal antibody

PCNA Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 60097-1-Ig

产品说明书

CloneNo. 10D10E11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

proliferating cell nuclear antigen, Cyclin, 10D10E11

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, C6 cells, ROS1728 cells, NIH/3T3 cells, 3T3-L1 cells, 4T1 cells, RAW 264.7 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman gliomas tissue, human breast cancer tissue, human liver cancer tissue, human placenta tissue, human tonsillitis tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, mouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy PCNA Ready-To-Use IHC Kit. PCNA primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 750 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60097-1-Ig targets PCNA in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rat, pig, rabbit, zebrafish
免疫原 PCNA fusion protein Ag7416 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proliferating cell nuclear antigen
别名 proliferating cell nuclear antigen, Cyclin, 10D10E11
计算分子量 29 kDa/31 kDa
观测分子量 36-38 kDa
GenBank蛋白编号BC000491
基因名称 PCNA
Gene ID (NCBI) 5111
RRIDAB_2236728
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP12004
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).

实验方案

Product Specific Protocols
WB protocol for PCNA antibody 60097-1-IgDownload protocol
IHC protocol for PCNA antibody 60097-1-IgDownload protocol
IF protocol for PCNA antibody 60097-1-IgDownload protocol
IP protocol for PCNA antibody 60097-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Commun

USP52 acts as a deubiquitinase and promotes histone chaperone ASF1A stabilization.

Authors - Shangda Yang
humanWB

Nat Commun

Cyclophilin J limits inflammation through the blockage of ubiquitin chain sensing.

Authors - Chunjie Sheng
pigWB

Microbiome

Antioxidant potential of Pediococcus pentosaceus strains from the sow milk bacterial collection in weaned piglets.

Authors - Leli Wang
mouse,ratWB,IF

Environ Pollut

Wnt10a downregulation contributes to MEHP-induced disruption of self-renewal and differentiation balance and proliferation inhibition in GC-1 cells: Insights from multiple transcriptomic profiling

Authors - Yifan Hong
mouseIHC

Biofabrication

Scale-out production of extracellular vesicles derived from natural killer cells via mechanical stimulation in a seesaw-motion bioreactor for cancer therapy.

Authors - Jianguo Wu
pigWB,IF,CoIP

Int J Biol Macromol

DNA ligase III mediates deoxynivalenol exposure-induced DNA damage in intestinal epithelial cells by regulating oxidative stress and interaction with PCNA

Authors - Xiaoyang Zhu
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