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- KD/KO Validated
CoraLite® Plus 750-conjugated PCNA Monoclonal antibody
PCNA Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, pig, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
10D10E11
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells |
For other applications, we recommend the unconjugated version of this antibody, 60097-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-60097 targets PCNA in WB applications and shows reactivity with human, mouse, pig, rat samples.
| Tested Applications | WB |
| Tested Reactivity | human, mouse, pig, rat |
| Immunogen | PCNA fusion protein Ag7416 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | proliferating cell nuclear antigen |
| Synonyms | Cyclin, PCNA |
| Calculated Molecular Weight | 29 kDa/31 kDa |
| Observed Molecular Weight | 36-38 kDa |
| GenBank Accession Number | BC000491 |
| Gene Symbol | PCNA |
| Gene ID (NCBI) | 5111 |
| Conjugate | CoraLite® Plus 750 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P12004 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 PCNA antibody CL750-60097 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |