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验证数据展示

  • IF Staining of mouse heart using 60097-1-Ig (same clone as 60097-1-PBS)

    IF Staining of mouse heart using 60097-1-Ig (same clone as 60097-1-PBS)

    E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

  • IHC staining of human gliomas using 60097-1-Ig (same clone as 60097-1-PBS)

    IHC staining of human gliomas using 60097-1-Ig (same clone as 60097-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCNA antibody included
  • IF Staining of HepG2 using 60097-1-Ig (same clone as 60097-1-PBS)

    IF Staining of HepG2 using 60097-1-Ig (same clone as 60097-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 60097-1-Ig (PCNA antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 60097-1-Ig (same clone as 60097-1-PBS)

    WB analysis of HEK-293 using 60097-1-Ig (same clone as 60097-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 60097-1-Ig (PCNA Antibody) at various dilution incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 60097-1-Ig (same clone as 60097-1-PBS)

    WB analysis of HeLa using 60097-1-Ig (same clone as 60097-1-PBS)

    Western blot analysis of PCNA in various cell lines using Proteintech antibody 60097-1-Ig at a dilution of 1:10000 (exposed for 2 seconds) incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 60097-1-Ig (same clone as 60097-1-PBS)

    IP experiment of HepG2 using 60097-1-Ig (same clone as 60097-1-PBS)

    IP result of anti-PCNA (IP:60097-1-Ig, 4ug; Detection:60097-1-Ig 1:300) with HepG2 cells lysate 3000ug. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 60097-1-Ig (same clone as 60097-1-PBS)

    WB analysis of HEK-293 using 60097-1-Ig (same clone as 60097-1-PBS)

    WB result of PCNA antibody (60097-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PCNA transfected HEK-293 cells. This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60097-1-Ig (same clone as 60097-1-PBS)

    IHC staining of human liver cancer using 60097-1-Ig (same clone as 60097-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCNA antibody included
  • IHC staining of human placenta using 60097-1-Ig (same clone as 60097-1-PBS)

    IHC staining of human placenta using 60097-1-Ig (same clone as 60097-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 60097-1-Ig (PCNA antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60097-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PCNA antibody included
  • Featured Product
  • KD/KO验证

PCNA Monoclonal antibody, PBS Only

PCNA Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 60097-1-PBS

产品说明书

CloneNo. 10D10E11

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

proliferating cell nuclear antigen, Cyclin, 10D10E11

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PCNA Ready-To-Use IHC Kit. PCNA primary antibody included.

产品信息

60097-1-PBS targets PCNA in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 PCNA fusion protein Ag7416 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 proliferating cell nuclear antigen
别名 proliferating cell nuclear antigen, Cyclin, 10D10E11
计算分子量 29 kDa/31 kDa
观测分子量 36-38 kDa
GenBank蛋白编号BC000491
基因名称 PCNA
Gene ID (NCBI) 5111
RRIDAB_2236728
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP12004
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Proliferating Cell Nuclear Antigen, commonly known as PCNA, is a protein that acts as a processivity factor for DNA polymerase δ in eukaryotic cells. This protein is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. PCNA induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. It has to be loaded onto DNA in order to be able to stimulate APEX2. PCNA protein is highly conserved during evolution; the deduced amino acid sequences of rat and human differ by only 4 of 261 amino acids. PCNA has been used as loading control for proliferating cells. The calculated molecular weight of PCNA is 29 kDa, but modified PCNA is 36kDa (PMID: 1358458).