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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Desmin Polyclonal antibody

Desmin Polyclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-16520

Print datasheet

Synonyms

DES

验证数据展示

  • IF Staining of mouse small intestine using CL488-16520

    IF Staining of mouse small intestine using CL488-16520

    Adult mouse small intestine was co-stained with desmin (CL488-16520, in cyan), E-cadherin (20874-1-AP, in red), and PCNA (60097-1-Ig, in green). Desmin marks the stromal cells and smooth muscle, E-cadherin marks the gut epithelial cells, and PCNA marks proliferating cells. The image was created in paid partnership with @Immunofluorescence on Instagram.

  • IF Staining of rat heart using CL488-16520

    IF Staining of rat heart using CL488-16520

    Immunofluorescent analysis of (4% PFA) fixed rat heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:100.

  • IF Staining of mouse heart using CL488-16520

    IF Staining of mouse heart using CL488-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.

  • IF Staining of C2C12 using CL488-16520

    IF Staining of C2C12 using CL488-16520

    Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CoraLite® Plus 488 Desmin antibody (CL488-16520) at dilution of 1:200.

  • IF Staining of mouse heart using CL488-16520

    IF Staining of mouse heart using CL488-16520

    E14.5 FFPE mouse heart stained for Desmin (green, Cat. No CL488-16520) and PCNA (red, Cat. No 60097-1-Ig). Desmin was conjugated to CoraLite-488 fluorescent dye and stains cardiomyocytes (heart muscle cells). PCNA stains proliferating cells, which are numerous in the developing heart. In this image, the four chambers of the heart can be easily visualized. Image credit: @Immunofluorescence on Instagram.

  • FC experiment of C2C12 using CL488-16520

    FC experiment of C2C12 using CL488-16520

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Desmin (CL488-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inmouse heart tissue, mouse small intestine tissue, rat heart tissue
Positive IF/ICC detected inC2C12 cells
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy Desmin Ready-To-Use IHC Kit. Desmin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16520-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

FCSee 1 publications below

产品信息

CL488-16520 targets Desmin in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Immunogen Desmin fusion protein Ag9742 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name desmin
Synonyms DES
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 52-53 kDa
GenBank Accession NumberBC032116
Gene Symbol Desmin
Gene ID (NCBI) 1674
RRIDAB_2883084
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17661
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Desmin antibody CL488-16520Download protocol
FC protocol for CL Plus 488 Desmin antibody CL488-16520Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseFC

J Vasc Res

Isolation and Cultivation of Vascular Smooth Muscle Cells from the Mouse Circle of Willis

Authors - Wei Chang
View Article