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验证数据展示

  • IF Staining of mouse heart using CL594-16520

    IF Staining of mouse heart using CL594-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

  • IF Staining of mouse heart using CL594-16520

    IF Staining of mouse heart using CL594-16520

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 Desmin antibody (CL594-16520) at dilution of 1:200.

  • FC experiment of C2C12 using CL594-16520

    FC experiment of C2C12 using CL594-16520

    1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Desmin (CL594-16520) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Desmin Polyclonal antibody

Desmin Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL594-16520

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Synonyms

DES

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive FC (Intra) detected inC2C12 cells
Planning an IHC experiment? We recommend our IHCeasy Desmin Ready-To-Use IHC Kit. Desmin primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 16520-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-16520 targets Desmin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen Desmin fusion protein Ag9742 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name desmin
Synonyms DES
Calculated Molecular Weight 470 aa, 54 kDa
Observed Molecular Weight 52-53 kDa
GenBank Accession NumberBC032116
Gene Symbol Desmin
Gene ID (NCBI) 1674
RRIDAB_2919835
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17661
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Desmin is the main intermediate filament protein in skeletal and cardiac muscle cells and is essential for both the structural integrity and the survival of muscle cells. As an abundant muscle-specific protein, desmin has been widely used as a marker of muscle derived tumors. Anti-desmin is also valuable in the differential diagnosis of tumors of uncertain origin.

实验方案

Product Specific Protocols
IF protocol for CL594 Desmin antibody CL594-16520Download protocol
FC protocol for CL594 Desmin antibody CL594-16520Download protocol
Standard Protocols
Click here to view our Standard Protocols