Desmin Polyclonal antibody
Desmin Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
FC, IF, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse heart tissue, human heart tissue, rat heart tissue |
Positive IP detected in | mouse heart tissue |
Positive IHC detected in | human heart tissue, human appendix tissue, human skeletal muscle tissue, human placenta tissue, human colon tissue, human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | C2C12 cells, mouse heart tissue, rat heart tissue |
Positive FC detected in | C2C12 cell |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:20000-1:100000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16520-1-AP targets Desmin in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | FC, IF, IHC, IP, WB, ELISA |
Cited Applications | IF, IHC, IP, WB |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, chicken |
Immunogen | Desmin fusion protein Ag9742 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | desmin |
Synonyms | Cell and organelle markers, CMD1I, CSM1, CSM2, Cytoskeleton Marker, DES, desmin |
Calculated Molecular Weight | 470 aa, 54 kDa |
Observed Molecular Weight | 52-53 kDa |
GenBank Accession Number | BC032116 |
Gene Symbol | DES |
Gene ID (NCBI) | 1674 |
RRID | AB_2292918 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P17661 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Desmin antibody 16520-1-AP | Download protocol |
IHC protocol for Desmin antibody 16520-1-AP | Download protocol |
IF protocol for Desmin antibody 16520-1-AP | Download protocol |
IP protocol for Desmin antibody 16520-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Theranostics miR-17-3p Contributes to Exercise-Induced Cardiac Growth and Protects against Myocardial Ischemia-Reperfusion Injury. | ||
Acta Physiol (Oxf) Podocyte apoptosis in diabetic nephropathy by BASP1 activation of the p53 pathway via WT1. | ||
Oxid Med Cell Longev Tanreqing Injection Regulates Cell Function of Hypoxia-Induced Human Pulmonary Artery Smooth Muscle Cells (HPASMCs) through TRPC1/CX3CL1 Signaling Pathway. | ||
J Invest Dermatol TRAF6 Activates Fibroblasts to Cancer-Associated Fibroblasts through FGF19 in Tumor Microenvironment to Benefit the Malignant Phenotype of Melanoma Cells. | ||
Aging (Albany NY) Vitellogenin 2 promotes muscle development and stimulates the browning of white fat | ||
Sci Rep MiR-146a-5p suppresses activation and proliferation of hepatic stellate cells in nonalcoholic fibrosing steatohepatitis through directly targeting Wnt1 and Wnt5a. |