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Lamin B1 Monoclonal antibody

Lamin B1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA
Cat No. 66095-1-Ig

产品说明书

CloneNo. 3C10G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat and More (5)

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA and More (2)

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
规格: 

-/ -


经过测试的应用

Positive WB detected inNCI-H1299 cells, multi-cells/tissue, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells, PC-12 cells, NIH/3T3 cells, 4T1cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue
Positive IF/ICC detected inHepG2 cells, HeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Lamin B1 Ready-To-Use IHC Kit. Lamin B1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:20000-1:100000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66095-1-Ig targets Lamin B1 in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, rabbit, chicken, zebrafish, bovine, hamster
免疫原 Lamin B1 fusion protein Ag20522 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lamin B1
别名 LMNB1, LMNB, LMN2, Lamin-B1, laminB1
计算分子量 66 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC012295
基因名称 Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_11232208
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP20700
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

实验方案

Product Specific Protocols
WB protocol for Lamin B1 antibody 66095-1-IgDownload protocol
IHC protocol for Lamin B1 antibody 66095-1-IgDownload protocol
IF protocol for Lamin B1 antibody 66095-1-IgDownload protocol
IP protocol for Lamin B1 antibody 66095-1-IgDownload protocol
FC protocol for Lamin B1 antibody 66095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIF

Nat Cell Biol

Ceramide-rich microdomains facilitate nuclear envelope budding for non-conventional exosome formation.

Authors - Subhash B Arya

J Hepatol

OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.

Authors - Weiqi Dai

Cell Rep Med

Management of prostate cancer by targeting 3βHSD1 after enzalutamide and abiraterone treatment.

Authors - Zejie Mei
humanWB

Nat Commun

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling

Authors - Maximilian Hirschenberger
humanWB

Nat Commun

FOXP3+ regulatory T cell perturbation mediated by the IFNγ-STAT1-IFITM3 feedback loop is essential for anti-tumor immunity

Authors - Xinnan Liu
humanWB

J Clin Invest

FAM117B promotes gastric cancer growth and drug resistance by targeting the KEAP1/NRF2 signaling pathway

Authors - Yunjiang Zhou
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