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Lamin B1 Monoclonal antibody 137 Publications

Mouse Monoclonal| Catalog number: 66095-1-Ig |CloneNo.: 3C10G12

Featured Product KD/KO validated

浓度: 1000 μg/ml

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Applications

Tested Applications:
FC, IF, IHC, IP, WB, ELISA
Application not tested? Please see our Guarantee


Cited Applications:
IF, IHC, IP, WB


Images See all

IF Staining of HepG2 using 66095-1-Ig

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

WB analysis of HepG2 using 66095-1-Ig

WB result of Lamin B1 antibody (66095-1-Ig, 1:300,000) with si-Control and si-Lamin B1 transfected HepG2 cells.

IF Staining of mouse eye using 66095-1-Ig

Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Lamin B1 antibody (66095-1-Ig, Clone: 3C10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

WB analysis of multi-cells/tissue using 66095-1-Ig

Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-Ig) at dilution 1:20000.

IHC staining of human pancreas cancer using 66095-1-Ig

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FC experiment of HeLa using 66095-1-Ig

1X10^6 HeLa cells were stained with 0.2ug Lamin B1 antibody (66095-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). FITC-Goat anti-Mouse IgG with dilution 1:100.

IHC staining of human pancreas cancer using 66095-1-Ig

Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP experiment of HeLa using 66095-1-Ig

IP Result of anti-Lamin B1 (IP:66095-1-Ig, 4ug; Detection:66095-1-Ig 1:20000) with HeLa cells lysate 3560ug.

Species Specificity:
human, mouse, rat
Cited Species:
human, mouse, Rabbit, rat, zebrafish
Positive WB detected in:
multi-cells/tissue, HepG2 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human pancreas cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HepG2 cells, mouse eye tissue, HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:10000-1:50000
IP : 0.5-4.0 ug for IP and 1:5000-1:50000 for WB
IHC : 1:500-1:2000
IF : 1:250-1:1000

Product Information

Source:
Mouse
Purification method:
Protein G purification
Isotype:
IgG1
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Lamin B1 fusion protein Ag20522
Full name:
lamin B1
Calculated molecular weight:
66 kDa
Observed molecular weight:
66-70 kDa
GenBank accession number:
BC012295
Gene ID (NCBI):
4001
Gene symbol
LMNB1
Synonyms
ADLD, lamin B1, LMN, LMN2, LMNB, LMNB1

Background

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

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