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验证数据展示

  • WB analysis using CL750-66095

    WB analysis using CL750-66095

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66095 (Lamin B1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated Lamin B1 Monoclonal antibody

Lamin B1 Monoclonal Antibody for WB
Cat No. CL750-66095

Print datasheet

CloneNo. 3C10G12

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB

Synonyms

LMNB1, LMNB, LMN2, Lamin-B1, laminB1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 750
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy Lamin B1 Ready-To-Use IHC Kit. Lamin B1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66095-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66095 targets Lamin B1 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat
Immunogen Lamin B1 fusion protein Ag20522 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name lamin B1
Synonyms LMNB1, LMNB, LMN2, Lamin-B1, laminB1
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC012295
Gene Symbol Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_3084888
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20700
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Lamin B1 antibody CL750-66095Download protocol
Standard Protocols
Click here to view our Standard Protocols