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验证数据展示

  • IF Staining of HepG2 using 66095-1-Ig (same clone as 66095-1-PBS)

    IF Staining of HepG2 using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:500 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • WB analysis of multi-cells/tissue using 66095-1-Ig (same clone as 66095-1-PBS)

    WB analysis of multi-cells/tissue using 66095-1-Ig (same clone as 66095-1-PBS)

    Western blot on multiple cells/tissues with anti-LMNB1 (66095-1-Ig) at dilution 1:20000. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • WB analysis using 66095-1-Ig (same clone as 66095-1-PBS)

    WB analysis using 66095-1-Ig (same clone as 66095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66095-1-Ig (Lamin B1 antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 66095-1-Ig (same clone as 66095-1-PBS)

    IHC staining of human pancreas cancer using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin B1 antibody included
  • IHC staining of human pancreas cancer using 66095-1-Ig (same clone as 66095-1-PBS)

    IHC staining of human pancreas cancer using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66095-1-Ig (Lamin B1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Lamin B1 antibody included
  • IP experiment of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    IP experiment of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    IP result of anti-Lamin B1 (IP:66095-1-Ig, 4ug; Detection:66095-1-Ig 1:20000) with HeLa cells lysate 3560ug. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse eye using 66095-1-Ig (same clone as 66095-1-PBS)

    IF Staining of mouse eye using 66095-1-Ig (same clone as 66095-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using Lamin B1 antibody (66095-1-Ig, Clone: 3C10G12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    FC experiment of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Lamin B1 (66095-1-Ig, Clone:3C10G12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    WB analysis of HeLa using 66095-1-Ig (same clone as 66095-1-PBS)

    WB result of Lamin B1 antibody (66095-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Lamin B1 transfected HeLa cells. This data was developed using the same antibody clone with 66095-1-PBS in a different storage buffer formulation.

  • Sample test of MP51325-1

    Sample test of MP51325-1

    Sample test of MP51325-1, Lamin B1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66095-1-PBS. Detection antibody: 66095-2-PBS.

  • Cytometric bead array standard curve of MP51325-1

    Cytometric bead array standard curve of MP51325-1

    Cytometric bead array standard curve of MP51325-1, Lamin B1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66095-1-PBS. Detection antibody: 66095-2-PBS. Standard:Ag20522. Range: 0.391-200 ng/mL.

  • Featured Product
  • KD/KO验证

Lamin B1 Monoclonal antibody, PBS Only (Capture)

Lamin B1 Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Cytometric bead array, Indirect ELISA, Sample test
Cat No. 66095-1-PBS

产品说明书

CloneNo. 3C10G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Cytometric bead array, Indirect ELISA, Sample test

别名

LMNB1, 3C10G12, laminB1, Lamin-B1, LMN2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy Lamin B1 Ready-To-Use IHC Kit. Lamin B1 primary antibody included.

产品信息

66095-1-PBS targets Lamin B1 as part of a matched antibody pair:

MP51325-1: 66095-1-PBS capture and 66095-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, IF-P, FC (Intra), IP, Cytometric bead array, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 Lamin B1 fusion protein Ag20522 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 lamin B1
别名 LMNB1, 3C10G12, laminB1, Lamin-B1, LMN2
计算分子量 66 kDa
观测分子量 66-70 kDa
GenBank蛋白编号BC012295
基因名称 Lamin B1
Gene ID (NCBI) 4001
ENSEMBL Gene IDENSG00000113368
RRIDAB_11232208
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP20700
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. This protein is not suitable for samples where the nuclear envelope has been removed.