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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 750-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2D4H5

Cat no : CL750-66009

Print datasheet

Synonyms

ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin

验证数据展示

  • WB analysis using CL750-66009

    WB analysis using CL750-66009

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66009 (Beta Actin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using CL750-66009

    WB analysis using CL750-66009

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66009 (Beta Actin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inmouse kidney tissue, mouse brain tissue, HeLa cells, NIH/3T3 cells, HSC-T6 cells, Jurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66009-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66009 targets Beta Actin in WB applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name actin, beta
Synonyms ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001101
Gene Symbol Beta Actin
Gene ID (NCBI) 60
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60709
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used "housekeeping gene" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.

 

What is the molecular weight of beta-actin?

42 kDa

 

What is the function of beta-actin?

Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).

 

What are the applications for beta-actin?

As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.

Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).

 

What are the post-transcriptional modifications (PTMs) to beta actin?

Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer's disease (PMID: 11246152). 


For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.



实验方案

Product Specific Protocols
WB protocol for CL Plus 750 Beta Actin antibody CL750-66009Download protocol
Standard Protocols
Click here to view our Standard Protocols