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  • Featured Product
  • KD/KO Validated

Beta Actin Monoclonal antibody, PBS Only

Beta Actin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, rabbit, canine, monkey, chicken, zebrafish, hamster, dog

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

2D4H5

Cat no : 66009-1-PBS

Print datasheet

Synonyms

ACTB, bate actin, β actin, Beta-actin, Actin, cytoplasmic 1

验证数据展示

  • IF Staining of MDCK using 66009-1-Ig (same clone as 66009-1-PBS)

    IF Staining of MDCK using 66009-1-Ig (same clone as 66009-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66009-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 cells using 66009-1-Ig (same clone as 66009-1-PBS)

    WB analysis of A549 cells using 66009-1-Ig (same clone as 66009-1-PBS)

    A549 cells (shcontrol and shRNA of Beta Actin) were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Mouse anti Pan-actin antibody) at dilution of 1:10000. This data was developed using the same antibody clone with 66009-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 66009-1-Ig (same clone as 66009-1-PBS)

    IP experiment of HeLa using 66009-1-Ig (same clone as 66009-1-PBS)

    IP result of anti-Beta Actin (IP:66009-1-Ig, 3ug; Detection:66009-1-Ig 1:10000) with HeLa cells lysate 3100ug. This data was developed using the same antibody clone with 66009-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66009-1-Ig (same clone as 66009-1-PBS)

    IHC staining of human kidney using 66009-1-Ig (same clone as 66009-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 66009-1-Ig at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66009-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ACTB antibody included
  • WB analysis using 66009-1-Ig (same clone as 66009-1-PBS)

    WB analysis using 66009-1-Ig (same clone as 66009-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Beta Actin antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66009-1-PBS in a different storage buffer formulation.

  • WB analysis using 66009-1-Ig (same clone as 66009-1-PBS)

    WB analysis using 66009-1-Ig (same clone as 66009-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66009-1-Ig (Beta Actin antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66009-1-PBS in a different storage buffer formulation.

  • IF Staining of MDCK using 66009-1-Ig (same clone as 66009-1-PBS)

    IF Staining of MDCK using 66009-1-Ig (same clone as 66009-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using Beta Actin antibody (66009-1-Ig, Clone: 2D4H5 ) at dilution of 1:3000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 66009-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66009-1-Ig (same clone as 66009-1-PBS)

    FC experiment of HeLa using 66009-1-Ig (same clone as 66009-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Beta Actin (66009-1-Ig, Clone:2D4H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66009-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.

产品信息

66009-1-PBS targets Beta Actin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit, canine, monkey, chicken, zebrafish, hamster, dog samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit, canine, monkey, chicken, zebrafish, hamster, dog
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name actin, beta
Synonyms ACTB, bate actin, β actin, Beta-actin, Actin, cytoplasmic 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberNM_001101
Gene Symbol Beta Actin
Gene ID (NCBI) 60
RRIDAB_2687938
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP60709
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates. Beta-actin (or β-actin) is a cytoskeletal actin isoform, highly conserved and ubiquitously expressed in eukaryotic organisms, making it a commonly used "housekeeping gene" in many laboratory techniques. It has been widely used as the internal control in RT-PCR and Western Blotting. The isotype of this antibody is IgG2b.

 

What is the molecular weight of beta-actin?

42 kDa

 

What is the function of beta-actin?

Actins are a key part of the cytoskeleton and are involved in cell motility, structure, and signaling, with beta-actin as part of the contractile apparatus. Beta-actin is located in the cytoplasm of non-muscle cells, particularly where the plasma membrane meets the cytoskeleton (PMID: 1993736) and is found co-distributed with alpha-actin (PMID: 9490838).

 

What are the applications for beta-actin?

As it is ubiquitously and constitutively expressed, beta-actin is a useful loading control in techniques such as RT-PCR and Western Blots to measure baseline expression and degradation. Beta-actin can be used as a reference gene to normalize mRNA or protein levels in quantitative studies of expression. Although some evidence suggests variation in tissue types and developing embryos (PMID: 23099774) if expression is stable across conditions, beta-actin is still considered by many to be a suitable gene for normalization.

Beyond its role as a housekeeping gene, it has been shown that beta-actin associates with endothelial nitric-oxide synthase (eNOS) and can therefore regulate nitric oxide activity in endothelial cells (PMID: 12734108).

 

What are the post-transcriptional modifications (PTMs) to beta actin?

Alterations to actin proteins can modulate their properties and function. While small molecules are known to alter actin, post-translational modifications such as phosphorylation and acetylation can also affect actin (PMID: 23195437). Modifications to actin have been associated with diseases including but not limited to age-related macular degeneration (PMID: 17652714), cardiovascular disease (PMID: 21232667), and Alzheimer's disease (PMID: 11246152). 


For murine tissue sample, conjugated antibody (HRP-66009) or rabbit antibody (20536-1-AP) is preferable.