GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for WB, IF/ICC, IF-P, IP, ELISA
Cat No. 50430-2-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

aequorea victoria, recombinant protein and More (2)

应用

WB, IF/ICC, IF-P, IP, ELISA and More (6)

eGFP, GFP, green, GFP-tag, eYFP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inrecombinant protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293T cells
Positive IF-P detected inmouse brain tissue
Positive IF/ICC detected inTransfected HEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

50430-2-AP targets GFP tag in WB, IHC, IF/ICC, IF-P, IP, CoIP, ChIP, RIP, IP-MS, ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.

经测试应用 WB, IF/ICC, IF-P, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP, RIP, IP-MS
经测试反应性 aequorea victoria, recombinant protein
文献引用反应性mouse, pig
免疫原 GFP tag fusion protein Ag2128 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 GFP tag
别名 eGFP, GFP, green, GFP-tag, eYFP
计算分子量 26 kDa
GenBank蛋白编号M62653
基因名称
Gene ID (NCBI)
RRIDAB_11042881
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a rabbit polyclonal antibody, generated against the full-length eGFP protein. It exhibits reactivity towards variants of Aequorea victoria GFP, including S65T-GFP, RS-GFP, YFP, CFP, and eGFP.


实验方案

Product Specific Protocols
WB protocol for GFP tag antibody 50430-2-APDownload protocol
IF protocol for GFP tag antibody 50430-2-APDownload protocol
IP protocol for GFP tag antibody 50430-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nat Biotechnol

Programmable protein expression using a genetically encoded m6A sensor

Authors - Bahjat F Marayati
WB

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Authors - Chuan Yang
WB

Cell

Phase separation drives RNA virus-induced activation of the NLRP6 inflammasome.

Authors - Chen Shen
WB,IF

Gastroenterology

PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis

Authors - Tian-Yi Jiang
WB

Nat Genet

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.

Authors - Margot A Cousin
WB,CoIP

Mol Plant

A TT1-SCE1 module integrates ubiquitination and SUMOylation to regulate heat tolerance in rice

Authors - Hong-Xiao Yu
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