GFP tag Polyclonal antibody

GFP tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


aequorea victoria, recombinant protein and More (8)


IF, IP, WB, ELISA and More (3)



Cat No : 50430-2-AP

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Positive WB detected inrecombinant protein, recombinant protein protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells


Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:20000 for WB
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery


50430-2-AP targets GFP tag in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with aequorea victoria, recombinant protein samples.

Tested Applications IF, IP, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, RIP, WB
Tested Reactivity aequorea victoria, recombinant protein
Cited Reactivitymouse, rat, Bacterial, chicken, Drosophila, E. coli, pig, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GFP tag fusion protein Ag2128
Full Name GFP tag
Calculated molecular weight 26 kDa
GenBank accession numberU73901
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.


Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. Green fluorescence protein(GFP) is a protein composed of 238 amino acid residues(26.9kDa) derived from the Jellyfish Aequorea victoria, which emits green light(emission peak at 509nm) when excited by blue light(excitation peak at 395nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP contains the double-amino-acid substitutions Phe-64 to Leu and Ser-65 to Thr(previously published as GFPmut1; PMID: 8707053). In contrast to wtGFP, EGFP has a single, strong, red-shifted excitation peak at 488nm. GFPmut1 fluoresces 35-fold more intensely than wtGFP when excited at 488nm, due to an increase in its extinction coefficient(Em). This antibody is a rabbit polyclonal antibody raised against full-length eGFP and reactive against all variants of Aequorea victoria GFP such as S65T-GFP, RS-GFP, YFP, CFP and eGFP.


Product Specific Protocols
WB protocol for GFP tag antibody 50430-2-APDownload protocol
IF protocol for GFP tag antibody 50430-2-APDownload protocol
IP protocol for GFP tag antibody 50430-2-APDownload protocol
Standard Protocols
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