验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells |
| Positive IF/ICC detected in | HeLa cells, MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| IF | See 1 publications below |
产品信息
CL594-60008 targets Beta Actin in WB, IF/ICC applications and shows reactivity with human, mouse, rat, plant, Zebrafish, canine samples.
| 经测试应用 | WB, IF/ICC Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat, plant, Zebrafish, canine |
| 文献引用反应性 | rat |
| 免疫原 | Beta Actin fusion protein Ag0297 种属同源性预测 |
| 宿主/亚型 | Mouse / IgM |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | actin, beta |
| 别名 | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, PS1TP5BP1, β actin |
| 计算分子量 | 375 aa, 42 kDa |
| GenBank蛋白编号 | BC002409 |
| 基因名称 | Beta Actin |
| Gene ID (NCBI) | 60 |
| RRID | AB_2883409 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Caprylic acid/ammonium sulfate precipitation |
| UNIPROT ID | P60709 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody is conjugated with CL594,Ex/Em 593 nm/614 nm.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL594 Beta Actin antibody CL594-60008 | Download protocol |
| IF protocol for CL594 Beta Actin antibody CL594-60008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Rep Translational enhancement of target endogenous mRNA in mammalian cells using programmable RNA-binding pentatricopeptide repeat proteins | ||
Neurosci Res Hippocampal Warburg effect Mediates Hydrogen Sulfide-Ameliorated Diabetes-Associated Cognitive Dysfunction: Involving Promotion of Hippocampal Synaptic Plasticity | ||
Proc Natl Acad Sci U S A Multifunctional biomolecular corona-inspired nanoremediation of antibiotic residues | ||
