HRP-conjugated Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for WB
Cat No. HRP-60008

产品说明书

CloneNo. 7D2C10

宿主/亚型

Mouse / IgM

种属反应性

human, mouse, rat, zebrafish, plant and More (5)

应用

WB and More (2)

Actin, ACTB, bate actin, β actin, Beta-actin

缓冲液配方:  PBS and Azide
PBS and Azide
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, multi-cells/tissue, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ACTB Ready-To-Use IHC Kit. ACTB primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-60008 targets Beta Actin in WB, IP, ELISA applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

经测试应用 WB Application Description
文献引用应用WB, IP, ELISA
经测试反应性 human, mouse, rat, zebrafish, plant
文献引用反应性human, mouse, rat, pig, canine, monkey, chicken, hamster
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 actin, beta
别名 Actin, ACTB, bate actin, β actin, Beta-actin
计算分子量 375 aa, 42 kDa
观测分子量 42 kDa
GenBank蛋白编号BC002409
基因名称 Beta Actin
Gene ID (NCBI) 60
RRIDAB_2819183
偶联类型 HRP
形式 Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP60709
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. This antibody can recognize all of actins. This antibody is conjugated with HRP.

Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.

实验方案

Product Specific Protocols
WB protocol for HRP Beta Actin antibody HRP-60008Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell

Biophysical forces mediated by respiration maintain lung alveolar epithelial cell fate

Authors - Kazushige Shiraishi

Gastroenterology

Dual inhibition of CDK4/6 and XPO1 induces senescence with acquired vulnerability to CRBN-based PROTAC drugs

Authors - Hui Wang
WB

J Hematol Oncol

METTL16 promotes liver cancer stem cell self-renewal via controlling ribosome biogenesis and mRNA translation

Authors - Meilin Xue
WB

Nat Neurosci

Human ALS/FTD brain organoid slice cultures display distinct early astrocyte and targetable neuronal pathology.

Authors - Kornélia Szebényi
mouseWB

Blood

Fetal liver hepcidin secures iron stores in utero.

Authors - Lara Kämmerer
humanWB

Nat Microbiol

Adeno-associated virus 2 bound to its cellular receptor AAVR.

Authors - Ran Zhang
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