Beta Actin Monoclonal antibody

Beta Actin Monoclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Mouse / IgM

Reactivity

human, mouse, rat, pig, plant, Zebrafish and More (10)

Applications

FC, IHC, WB, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

7D2C10

Cat No : 60008-1-Ig

Print datasheet

Synonyms

Beta actin



经过测试的应用

Positive WB detected inmulti-cells/tissue, HeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, rice whole plant tissue, arabidopsis whole plant tissue
Positive IHC detected inhuman colon tissue, human brain tissue, human kidney tissue, human lung tissue, human spleen tissue, human ovary tissue, human testis tissue, human placenta tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

产品信息

60008-1-Ig targets Beta Actin in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, plant, Zebrafish samples.

Tested Applications FC, IHC, WB, ELISA
Cited Applications CoIP, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat, pig, plant, Zebrafish
Cited ReactivityArabidopsis, Artemia sinica, Caenorhabditis elegans, canine, cat, cattle, chicken, Dendrobium nobile, duck, gecko
Host / Isotype Mouse / IgM
Class Monoclonal
Type Antibody
Immunogen Beta Actin fusion protein Ag0297 种属同源性预测
Full Name actin, beta
Synonyms Beta actin
Calculated molecular weight 375 aa, 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002409
Gene symbol ACTB
UniprotP60709
Gene ID (NCBI) 60
RRIDAB_2289225
Conjugate Unconjugated
Form Liquid
Purification Method Caprylic acid/ammonium sulfate precipitation
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha (ACTC1, cardiac muscle 1), alpha 1 (ACTA1, skeletal muscle) and 2 (ACTA2, aortic smooth muscle), beta (ACTB), gamma 1 (ACTG1) and 2 (ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2 (rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 37-40 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody can recognize all the actins.

实验方案

Product Specific Protocols
WB protocol for Beta Actin antibody 60008-1-IgDownload protocol
IHC protocol for Beta Actin antibody 60008-1-IgDownload protocol
IF protocol for Beta Actin antibody 60008-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nature

A phase-separated nuclear GBPL circuit controls immunity in plants.

Authors - Shuai Huang
humanIF

Nat Methods

Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).

Authors - Marine H Laporte
humanWB

Nat Genet

Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells.

Authors - Gerard L Brien
humanWB

Cancer Cell

iASPP Is an Antioxidative Factor and Drives Cancer Growth and Drug Resistance by Competing with Nrf2 for Keap1 Binding.

Authors - Wenjie Ge
humanWB

Cancer Cell

Reuterin in the healthy gut microbiome suppresses colorectal cancer growth through altering redox balance.

Authors - Hannah N Bell
humanWB

Cell Metab

PMID: 31708445

Authors - Nupur K Das