Beta Actin Monoclonal antibody
Beta Actin Monoclonal Antibody for WB, IHC, ELISA
Host / Isotype
Mouse / IgM
Reactivity
human, mouse, rat, plant, Zebrafish and More (8)
Applications
WB, IHC, ELISA and More (3)
Conjugate
Unconjugated
3096
CloneNo.
7D2C10
验证数据展示
经过测试的应用
Positive WB detected in | multi-cells/tissue, HeLa cells, MCF-7 cells, HEK-293 cells, A549 cells, rice whole plant tissue, arabidopsis whole plant tissue |
Positive IHC detected in | human colon tissue, human brain tissue, human kidney tissue, human lung tissue, human spleen tissue, human ovary tissue, human testis tissue, human placenta tissue, human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
60008-1-Ig targets Beta Actin in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, plant, Zebrafish samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC, IF, IP, CoIP |
Tested Reactivity | human, mouse, rat, plant, Zebrafish |
Cited Reactivity | human, rat, chicken, goat, yeast, tick, arabidopsis, terminalia bellirica, rare minnow, cat |
Immunogen | Beta Actin fusion protein Ag0297 种属同源性预测 |
Host / Isotype | Mouse / IgM |
Class | Monoclonal |
Type | Antibody |
Full Name | actin, beta |
Synonyms | ACTB, Actin, actin, beta, Actin, cytoplasmic 1, B actin, Beta actin, F actin, β actin |
Calculated Molecular Weight | 375 aa, 42 kDa |
Observed Molecular Weight | 42 kDa |
GenBank Accession Number | BC002409 |
Gene Symbol | Beta Actin |
Gene ID (NCBI) | 60 |
RRID | AB_2289225 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Caprylic acid/ammonium sulfate precipitation |
UNIPROT ID | P60709 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Beta Actin antibody 60008-1-Ig | Download protocol |
IHC protocol for Beta Actin antibody 60008-1-Ig | Download protocol |
IF protocol for Beta Actin antibody 60008-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Res Mitochondria-localized cGAS suppresses ferroptosis to promote cancer progression | ||
Gastroenterology PTEN deficiency facilitates exosome secretion and metastasis in cholangiocarcinoma by impairing TFEB-mediated lysosome biogenesis | ||
Nat Methods Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM). | ||
Nat Genet Simultaneous disruption of PRC2 and enhancer function underlies histone H3.3-K27M oncogenic activity in human hindbrain neural stem cells. |