DYKDDDDK tag Recombinant antibody (Binds to FLAG® tag epitope)
DYKDDDDK tag Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
recombinant protein
Applications
WB, IF/ICC, FC (Intra), IP, ELISA
Conjugate
Unconjugated
CloneNo.
240568A11
验证数据展示
经过测试的应用
Positive WB detected in | TDP-43-flag Transfected HEK-293T cells |
Positive IP detected in | Transfected HEK-293 cells |
Positive IF/ICC detected in | Transfected HEK-293T cells |
Positive FC (Intra) detected in | HEK-293T transfected with TDP-43-flag |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
The immunogen of 80801-2-RR is DYKDDDDK tag Fusion Protein expressed in E. coli.
Tested Applications | WB, IF/ICC, FC (Intra), IP, ELISA Application Description |
Tested Reactivity | recombinant protein |
Immunogen | DYKDDDDK tag fusion protein Ag2329 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | DYKDDDDK tag |
Synonyms | 3Flag, Flag, DDDDKtag, DDDDK tag, DDDDK |
Gene Symbol | |
Gene ID (NCBI) | |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
IF protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
IP protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
FC protocol for DYKDDDDK tag antibody 80801-2-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |