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Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

WB, IHC, IF/ICC, FC, FC (Intra), IP

Conjugate

Unconjugated

CloneNo.

240953C9

Cat no : 98136-1-RR

Print datasheet

Synonyms

Rabbit IgG Isotype Control, Rabbit IgG isotype, Rabbit IgG Control, Rabbit IgG

验证数据展示

  • FC experiment of human PBMCs using 98136-1-RR

    FC experiment of human PBMCs using 98136-1-RR

    1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed.

  • IP experiment of HepG2 using 98136-1-RR

    IP experiment of HepG2 using 98136-1-RR

    IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug.

  • IF Staining of HeLa using 98136-1-RR

    IF Staining of HeLa using 98136-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control.

  • FC experiment of SH-SY5Y using 98136-1-RR

    FC experiment of SH-SY5Y using 98136-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 98136-1-RR

    WB analysis using 98136-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 98136-1-RR

    IHC staining of mouse colon using 98136-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected in
Positive IP detected in
Positive IHC detected in
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Positive FC (Intra) detected in
Positive FC detected in

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP applications and shows reactivity with n/a samples.

Tested Applications WB, IHC, IF/ICC, FC, FC (Intra), IP Application Description
Tested Reactivity n/a
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IgG Isotype Control
Synonyms Rabbit IgG Isotype Control, Rabbit IgG isotype, Rabbit IgG Control, Rabbit IgG
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

实验方案

Product Specific Protocols
WB protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IHC protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IF protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IP protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols