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Cadherin-17 Recombinant antibody

Cadherin-17 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

240681B11

Cat no : 83793-5-RR

Print datasheet

Synonyms

CDH17, HPT1, HPT 1, CDH16, Cadherin 17

验证数据展示

  • WB analysis of mouse colon using 83793-5-RR

    WB analysis of mouse colon using 83793-5-RR

    mouse colon tissue was subjected to SDS PAGE followed by western blot with 83793-5-RR (Cadherin-17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 83793-5-RR

    IHC staining of mouse colon using 83793-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDH17 antibody included
  • IHC staining of mouse colon using 83793-5-RR

    IHC staining of mouse colon using 83793-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83793-5-RR (Cadherin-17 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CDH17 antibody included
  • Affinity and Kinetic Characterization of 83793-5-RR

    Affinity and Kinetic Characterization of 83793-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83793-5-RR against Human Cadherin-17 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inmouse colon tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CDH17 Ready-To-Use IHC Kit. CDH17 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83793-5-RR is Cadherin-17 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Cadherin-17 fusion protein Ag19488 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 17, LI cadherin (liver-intestine)
Synonyms CDH17, HPT1, HPT 1, CDH16, Cadherin 17
Calculated Molecular Weight 832 aa, 92 kDa
Observed Molecular Weight120 kDa
GenBank Accession NumberBC113464
Gene Symbol Cadherin-17
Gene ID (NCBI) 1015
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ12864
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherin-17, also known as LI-cadherin, is a member of the cadherin superfamily. Cadherins are transmembrane proteins that mediates cell-cell adhesion, plays important roles during embryogenesis, and is crucial for tissue morphogenesis and maintenance. Cadherin-17 is a component of the gastrointestinal tract and pancreatic ducts and is involved in intestinal peptide transport. It may also play a role in the morphological organization of liver and intestine.

实验方案

Product Specific Protocols
WB protocol for Cadherin-17 antibody 83793-5-RRDownload protocol
IHC protocol for Cadherin-17 antibody 83793-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols