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×
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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of human PBMCs using 98136-1-RR (same clone as 98136-1-PBS)

    FC experiment of human PBMCs using 98136-1-RR (same clone as 98136-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 98136-1-RR (same clone as 98136-1-PBS)

    IP experiment of HepG2 using 98136-1-RR (same clone as 98136-1-PBS)

    IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

    IF Staining of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 98136-1-RR (same clone as 98136-1-PBS)

    FC experiment of SH-SY5Y using 98136-1-RR (same clone as 98136-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • WB analysis using 98136-1-RR (same clone as 98136-1-PBS)

    WB analysis using 98136-1-RR (same clone as 98136-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 98136-1-RR (same clone as 98136-1-PBS)

    IHC staining of mouse colon using 98136-1-RR (same clone as 98136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

Rabbit IgG Isotype Control Recombinant Antibody, PBS Only

IgG Isotype Control Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP
Cat No. 98136-1-PBS

产品说明书

CloneNo. 240953C9

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

WB, IHC, IF/ICC, FC, FC (Intra), IP

别名

Rabbit Isotype Control, Rabbit IgG Isotype Control, Rabbit IgG Control, Rabbit IgG

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

98136-1-PBS targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP applications and shows reactivity with n/a samples.

经测试应用 WB, IHC, IF/ICC, FC, FC (Intra), IP Application Description
经测试反应性 n/a
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IgG Isotype Control
别名 Rabbit Isotype Control, Rabbit IgG Isotype Control, Rabbit IgG Control, Rabbit IgG
基因名称
Gene ID (NCBI)
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.