• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Rabbit IgG Isotype Control Recombinant Antibody, PBS Only

IgG Isotype Control Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

WB, IHC, IF/ICC, FC, FC (Intra), IP

Conjugate

Unconjugated

CloneNo.

240953C9

Cat no : 98136-1-PBS

Print datasheet

Synonyms

Rabbit Isotype Control, Rabbit IgG Isotype Control, Rabbit IgG Control, Rabbit IgG

验证数据展示

  • FC experiment of human PBMCs using 98136-1-RR (same clone as 98136-1-PBS)

    FC experiment of human PBMCs using 98136-1-RR (same clone as 98136-1-PBS)

    1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 98136-1-RR (same clone as 98136-1-PBS)

    IP experiment of HepG2 using 98136-1-RR (same clone as 98136-1-PBS)

    IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

    IF Staining of HeLa using 98136-1-RR (same clone as 98136-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 98136-1-RR (same clone as 98136-1-PBS)

    FC experiment of SH-SY5Y using 98136-1-RR (same clone as 98136-1-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • WB analysis using 98136-1-RR (same clone as 98136-1-PBS)

    WB analysis using 98136-1-RR (same clone as 98136-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 98136-1-RR (same clone as 98136-1-PBS)

    IHC staining of mouse colon using 98136-1-RR (same clone as 98136-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98136-1-PBS in a different storage buffer formulation.

产品信息

98136-1-PBS targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP applications and shows reactivity with n/a samples.

Tested Applications WB, IHC, IF/ICC, FC, FC (Intra), IP Application Description
Tested Reactivity n/a
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IgG Isotype Control
Synonyms Rabbit Isotype Control, Rabbit IgG Isotype Control, Rabbit IgG Control, Rabbit IgG
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.