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验证数据展示

  • FC experiment of human PBMCs using 98136-1-RR

    FC experiment of human PBMCs using 98136-1-RR

    1x10^6 human PBMCs were surface stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red in histogram), or 0.2 ug Anti-Human CD4 Rabbit Recombinant Antibody (98042-1-RR) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (green in histogram), or unstained (blue in histogram). Cells were not fixed.

  • IP experiment of HepG2 using 98136-1-RR

    IP experiment of HepG2 using 98136-1-RR

    IP result of anti-IgG Isotype Control (IP:98136-1-RR, 4ug; Detection:98136-1-RR 1:0) with HepG2 cells lysate 2000 ug.

  • IF Staining of HeLa using 98136-1-RR

    IF Staining of HeLa using 98136-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using IgG Isotype Control antibody (98136-1-RR, Clone: 240953C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). NOL11 Recombinant antibody (83391-6-RR) is a positive control.

  • FC experiment of SH-SY5Y using 98136-1-RR

    FC experiment of SH-SY5Y using 98136-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.2 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone:240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or 0.2 ug PGF Recombinant antibody (83170-4-RR, Clone:230532H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (green), or unstain (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 98136-1-RR

    WB analysis using 98136-1-RR

    HeLa cells were subjected to SDS PAGE followed by western blot with 98136-1-RR (IgG Isotype Control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 98136-1-RR

    IHC staining of mouse colon using 98136-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 98136-1-RR (IgG Isotype Control antibody) at dilution of 2 ug/mL or 83793-5-RR (Cadherin-17 Recombinant antibody) or unstained (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC, FC (Intra), IP
Cat No. 98136-1-RR

产品说明书

CloneNo. 240953C9

宿主/亚型

Rabbit / IgG

种属反应性

n/a

应用

WB, IHC, IF/ICC, FC, FC (Intra), IP

别名

Rabbit IgG Isotype Control, Rabbit IgG isotype, Rabbit IgG Control, Rabbit IgG

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected in
Positive IP detected in
Positive IHC detected in
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
Positive FC (Intra) detected in
Positive FC detected in

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98136-1-RR targets IgG Isotype Control in WB, IHC, IF/ICC, FC, FC (Intra), IP applications and shows reactivity with n/a samples.

经测试应用 WB, IHC, IF/ICC, FC, FC (Intra), IP Application Description
经测试反应性 n/a
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 IgG Isotype Control
别名 Rabbit IgG Isotype Control, Rabbit IgG isotype, Rabbit IgG Control, Rabbit IgG
基因名称
Gene ID (NCBI)
RRIDAB_3672282
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

实验方案

Product Specific Protocols
WB protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IHC protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IF protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
IP protocol for IgG Isotype Control antibody 98136-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols