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Species Reactivity
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CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody

IgG Isotype Control Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

n/a

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

240953C9

Cat no : CL647-98136

Print datasheet

Synonyms

Rabbit IgG Isotype Control, Rabbit IgG Isotype, Rabbit IgG, 240953C9

验证数据展示

  • FC experiment of human peripheral blood leukocytes using CL647-98136

    FC experiment of human peripheral blood leukocytes using CL647-98136

    1x10^6 human peripheral blood leukocytes were unstained(left, blue in histogram) or surface stained with 0.25 ug CoraLite® Plus 647 Rabbit IgG Isotype Control Recombinant Antibody (CL647-98136, Clone:240953C9)(mid, red in histogram), or 0.25 ug CoraLite® Plus 647 Anti-Human CD45 Rabbit Recombinant Antibody (CL647-98117, Clone: 241670E10) (right, green in histogram). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

经过测试的应用

Positive FC detected in
For other applications, we recommend the unconjugated version of this antibody, 98136-1-PBS

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-98136 targets IgG Isotype Control in FC applications and shows reactivity with n/a samples.

Tested Applications FC Application Description
Tested Reactivity n/a
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name IgG Isotype Control
Synonyms Rabbit IgG Isotype Control, Rabbit IgG Isotype, Rabbit IgG, 240953C9
Gene Symbol
Gene ID (NCBI)
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.09% sodium azide.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

This product is an isotype control antibody, which is used to estimate the non-specific binding of target primary antibodies due to Fc receptor binding or other protein-protein interactions. An isotype control antibody should have the same immunoglobulin type and be used at the same concentration as the test antibody.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IgG Isotype Control antibody CL647-98136Download protocol
Standard Protocols
Click here to view our Standard Protocols