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Acetyl-Histone H2A (Lys9) Recombinant antibody

Acetyl-Histone H2A (Lys9) Recombinant Antibody for WB, IHC, IF/ICC, Dot Blot, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Dot Blot, ELISA

Conjugate

Unconjugated

CloneNo.

230349B1

Cat No : 83041-1-RR

Print datasheet

Synonyms

H2AK9ac, HIST1H2AE, H2AC8, H2AC4, H2A/a

验证数据展示

  • Dot Blot experiment of peptide using 83041-1-RR

    Dot Blot experiment of peptide using 83041-1-RR

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.Column 1: H2AEK9Ac. Column 2: Unmodified H2AEK9. Column 3: H2AEK5Ac. Column 4: Unmodified H2AEK5. Column 5: H2AEK13Ac. Column 6: Unmodified H2AEK13. Column 7: H2AEK15Ac. Column 8: Unmodified H2AEK15. Column 9: Blank(PBS).

  • WB analysis of NIH/3T3 using 83041-1-RR

    WB analysis of NIH/3T3 using 83041-1-RR

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse lung using 83041-1-RR

    IHC staining of mouse lung using 83041-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse lung using 83041-1-RR

    IHC staining of mouse lung using 83041-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 83041-1-RR

    IF Staining of HeLa using 83041-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inTrichostatin A treated NIH/3T3 cells
Positive IHC detected inmouse lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83041-1-RR targets Acetyl-Histone H2A (Lys9) in WB, IHC, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, Dot Blot, ELISA Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H2ae
Synonyms H2AK9ac, HIST1H2AE, H2AC8, H2AC4, H2A/a
Calculated Molecular Weight 14 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC093836
Gene Symbol HIST1H2AE
Gene ID (NCBI) 3012
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04908
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone H2A is a core component of nucleosome. Histone variants contribute to chromatin complexity by creating specialized nucleosomes. Within nucleosomes, either one canonical H2A or both of them can be exchanged with a particular variant (heterotypic and homotypic nucleosomes, respectively), and such changes can have profound influences on nucleosome stability and biological outcome.

实验方案

Product Specific Protocols
WB protocol for Acetyl-Histone H2A (Lys9) antibody 83041-1-RRDownload protocol
IHC protocol for Acetyl-Histone H2A (Lys9) antibody 83041-1-RRDownload protocol
IF protocol for Acetyl-Histone H2A (Lys9) antibody 83041-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols