MYC tag Polyclonal antibody

MYC tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (4)

Applications

IF, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 16286-1-AP

Print datasheet

Synonyms

EQKLISEEDL, myc, MYC tag, myc-tag



经过测试的应用

Positive WB detected inRecombinant protein protein, Recombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells, MCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 16286-1-AP is MYC tag Fusion Protein expressed in E. coli.

Tested Applications IF, IP, WB, ELISA
Cited Applications CoIP, FC, IF, IHC, IP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYC tag fusion protein Ag9409
Full Name MYC tag
Gene symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.

What is the molecular weight of myc?

1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).

What are the applications for myc tag?

The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106). 

What is the structure of myc tag?

The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.


实验方案

Product Specific Protocols
WB protocol for MYC tag antibody 16286-1-APDownload protocol
IF protocol for MYC tag antibody 16286-1-APDownload protocol
IP protocol for MYC tag antibody 16286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

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