MYC tag Polyclonal antibody

MYC tag Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 16286-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein and More (2)

应用

WB, IF/ICC, IP, ELISA and More (4)

myc, EQKLISEEDL, myc-tag

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inRecombinant protein protein, Recombinant protein
Positive IP detected inTransfected HEK-293 cells
Positive IF/ICC detected inTransfected HEK-293 cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16286-1-AP targets MYC tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ChIP
经测试反应性 recombinant protein
文献引用反应性human, mouse
免疫原 MYC tag fusion protein Ag9409 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Myc tag
别名 myc, EQKLISEEDL, myc-tag
基因名称 Myc tag
Gene ID (NCBI) 99
RRIDAB_11182162
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.

What is the molecular weight of myc?

1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).

What are the applications for myc tag?

The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106). 

What is the structure of myc tag?

The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.


实验方案

Product Specific Protocols
WB protocol for MYC tag antibody 16286-1-APDownload protocol
IF protocol for MYC tag antibody 16286-1-APDownload protocol
IP protocol for MYC tag antibody 16286-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
WB,CoIP

Signal Transduct Target Ther

Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis

Authors - Chuan Yang

Nat Microbiol

Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry.

Authors - Hua Zhang

Drug Resist Updat

MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance

Authors - GuoSheng Wang

Cell Stem Cell

A primate-specific endogenous retroviral envelope protein sequesters SFRP2 to regulate human cardiomyocyte development

Authors - Ran Zhang
CoIP

Nat Commun

The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells

Authors - Xiaofeng Liao
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