验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein protein, Recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF/ICC detected in | Transfected HEK-293 cells, MCF-7 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16286-1-AP targets MYC tag in WB, IHC, IF/ICC, IP, CoIP, ChIP, RIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP, CoIP, ChIP, RIP |
| 经测试反应性 | recombinant protein |
| 文献引用反应性 | human, mouse, pig |
| 免疫原 | MYC tag fusion protein Ag9409 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Myc tag |
| 别名 | myc, EQKLISEEDL, myc-tag |
| 基因名称 | Myc tag |
| Gene ID (NCBI) | 99 |
| RRID | AB_11182162 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.
What is the molecular weight of myc?
1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).
What are the applications for myc tag?
The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106).
What is the structure of myc tag?
The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MYC tag antibody 16286-1-AP | Download protocol |
| IF protocol for MYC tag antibody 16286-1-AP | Download protocol |
| IP protocol for MYC tag antibody 16286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
Nat Microbiol Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry. | ||
Drug Resist Updat MYC expression and fatty acid oxidation in EGFR-TKI acquired resistance | ||
Cell Stem Cell A primate-specific endogenous retroviral envelope protein sequesters SFRP2 to regulate human cardiomyocyte development | ||
Nat Commun The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells |
