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Histone-H3 Polyclonal antibody 358 Publications

Rabbit Polyclonal| Catalog number: 17168-1-AP

浓度: 600 μg/ml

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Applications

Tested Applications:
FC, IF, IHC, WB,ELISA
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Cited Applications:
ChIP, IF, WB


Images See all

WB analysis of HEK-293 using 17168-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

FC experiment of HeLa using 17168-1-AP

1X10^6 HeLa cells were stained with 0.20ug Histone-H3 antibody (17168-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.

IHC staining of human breast cancer using 17168-1-AP

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17168-1-AP (Histone-H3 Antibody) at dilution of 1:50 (under 40x lens).

IHC staining of human skin cancer using 17168-1-AP

Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17168-1-AP (Histone-H3 Antibody) at dilution of 1:50 (under 40x lens).

IF Staining of HEK-293 using 17168-1-AP

Immunofluorescent analysis of HEK-293 cells using 17168-1-AP (Histone-H3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Species Specificity:
human, mouse, rat
Cited Species:
Arabidopsis, Bovine, canine, fish, frog, human, monkey, mouse, pig, rat
Positive WB detected in:
HEK-293 cells, mouse brain tissue, mouse skeletal muscle tissue, mouse liver tissue, A549 cells
Positive IHC detected in:
human skin cancer tissue, human breast cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HEK-293 cells, HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:6000
IHC : 1:50-1:200
IF : 1:20-1:200

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
Histone-H3 fusion protein Ag10644
Full name:
histone cluster 2, H3a
Calculated molecular weight:
136 aa, 15 kDa
Observed molecular weight:
15-17 kDa
GenBank accession number:
BC015544
Gene ID (NCBI):
333932
Gene symbol
HIST2H3A
Synonyms
H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o

Background

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

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