|Positive WB detected in||HEK-293 cells, mouse liver tissue, mouse brain tissue, A549 cells, mouse skeletal muscle tissue, Hela cells, HepG cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue|
|Positive IF detected in||HEK-293 cells, HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:16000|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 17168-1-AP is Histone-H3 Fusion Protein expressed in E. coli.
|Tested Applications||FC, IF, WB, ELISA|
|Cited Applications||ChIP, IF, IP, WB|
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, Arabidopsis, Bovine, canine, chicken, fish, frog, goat, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Histone-H3 fusion protein Ag10644|
|Full Name||histone cluster 2, H3a|
|Calculated molecular weight||136 aa, 15 kDa|
|Observed molecular weight||15-17 kDa|
|GenBank accession number||BC015544|
|Uniprot||Q71DI3||Gene ID (NCBI)||333932|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.
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