Histone-H3 Polyclonal antibody

Histone-H3 Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (8)

Applications

FC, IF, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 17168-1-AP

Print datasheet

Synonyms

H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone, histone 3, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o, Histone-H3



经过测试的应用

Positive WB detected inHEK-293 cells, mouse liver tissue, mouse brain tissue, A549 cells, mouse skeletal muscle tissue, Hela cells, HepG cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IF detected inHEK-293 cells, HeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®650 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

产品信息

The immunogen of 17168-1-AP is Histone-H3 Fusion Protein expressed in E. coli.

Tested Applications FC, IF, WB, ELISA
Cited Applications ChIP, IF, IP, WB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, Arabidopsis, Bovine, canine, chicken, fish, frog, goat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone-H3 fusion protein Ag10644
Full Name histone cluster 2, H3a
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC015544
Gene symbol HIST2H3A
UniprotQ71DI3
Gene ID (NCBI) 333932
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

实验方案

Product Specific Protocols
WB protocol for Histone-H3 antibody 17168-1-APDownload protocol
IHC protocol for Histone-H3 antibody 17168-1-APDownload protocol
IF protocol for Histone-H3 antibody 17168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB

Biomed Pharmacother

CP-25 combined with MTX/ LEF ameliorates the progression of adjuvant-induced arthritis by the inhibition on GRK2 translocation.

Authors - Xuezhi Yang
humanWB

Theranostics

Adipocytes fuel gastric cancer omental metastasis via PITPNC1-mediated fatty acid metabolic reprogramming.

Authors - Yujing Tan
humanWB

Free Radic Biol Med

5-(3,4-Difluorophenyl)-3-(6-methylpyridin-3-yl)-1,2,4-oxadiazole (DDO-7263), a Novel Nrf2 Activator Targeting Brain Tissue, Protects Against MPTP-induced Subacute Parkinson's Disease in Mice by Inhibiting the NLRP3 Inflammasome and Protects PC12 Cells Against Oxidative Stress.

Authors - Li-Li Xu
ratWB

Iran J Basic Med Sci

Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress.

Authors - Yeying Wang
mouseWB

Mol Immunol

Blockade of YAP alleviates hepatic fibrosis through accelerating apoptosis and reversion of activated hepatic stellate cells.

Authors - Hai-Xia Yu
humanWB

FEBS J

Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis.

Authors - Yi-Xuan Chen