Histone-H3 Polyclonal antibody
Histone-H3 Polyclonal Antibody for FC, IF, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (8)
Applications
FC, IF, WB, ELISA and More (2)
Conjugate
Unconjugated
466
验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, mouse liver tissue, mouse brain tissue, A549 cells, mouse skeletal muscle tissue, Hela cells, HepG cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue |
| Positive IF detected in | HEK-293 cells, HeLa cells |
| Positive FC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunofluorescence (IF) | IF : 1:20-1:200 |
| Sample-dependent, check data in validation data gallery | |
产品信息
The immunogen of 17168-1-AP is Histone-H3 Fusion Protein expressed in E. coli.
| Tested Applications | FC, IF, WB, ELISA |
| Cited Applications | ChIP, IF, IP, WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, Arabidopsis, Bovine, canine, chicken, fish, frog, goat, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Histone-H3 fusion protein Ag10644 |
| Full Name | histone cluster 2, H3a |
| Calculated molecular weight | 136 aa, 15 kDa |
| Observed molecular weight | 15-17 kDa |
| GenBank accession number | BC015544 |
| Gene symbol | HIST2H3A |
| Uniprot | Q71DI3 | Gene ID (NCBI) | 333932 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Histone-H3 antibody 17168-1-AP | Download protocol |
| IHC protocol for Histone-H3 antibody 17168-1-AP | Download protocol |
| IF protocol for Histone-H3 antibody 17168-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biomed Pharmacother CP-25 combined with MTX/ LEF ameliorates the progression of adjuvant-induced arthritis by the inhibition on GRK2 translocation. | ||
Theranostics Adipocytes fuel gastric cancer omental metastasis via PITPNC1-mediated fatty acid metabolic reprogramming. | ||
Free Radic Biol Med 5-(3,4-Difluorophenyl)-3-(6-methylpyridin-3-yl)-1,2,4-oxadiazole (DDO-7263), a Novel Nrf2 Activator Targeting Brain Tissue, Protects Against MPTP-induced Subacute Parkinson's Disease in Mice by Inhibiting the NLRP3 Inflammasome and Protects PC12 Cells Against Oxidative Stress. | ||
Iran J Basic Med Sci Tempol relieves lung injury in a rat model of chronic intermittent hypoxia via suppression of inflammation and oxidative stress. | ||
Mol Immunol Blockade of YAP alleviates hepatic fibrosis through accelerating apoptosis and reversion of activated hepatic stellate cells. | ||
FEBS J Diminished membrane recruitment of Akt is instrumental in alcohol-associated osteopenia via the PTEN/Akt/GSK-3β/β-catenin axis. |
