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IHCeasy Histone-H3 Ready-To-Use IHC Kit

Histone-H3 Ready-to-use reagent kit for IHC.

Reactivity

Human, Mouse, Rat

Sample Type

FFPE tissue

Cat No : KHC0568

Synonyms

H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone, histone 3, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o, Histone-H3

Validation Data Gallery

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0568 (Histone-H3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using KHC0568 (Histone-H3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0568 (Histone-H3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using KHC0568 (Histone-H3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using KHC0568 (Histone-H3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using KHC0568 (Histone-H3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0568 (Histone-H3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using KHC0568 (Histone-H3 IHC Kit).

  • Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0568 (Histone-H3 IHC Kit).

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using KHC0568 (Histone-H3 IHC Kit).

  • Components in IHCeasy ready-to-use IHC kit.

Product Information

KHC0568 is a ready-to-use IHC kit for staining of Histone-H3. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.

Product name IHCeasy Histone-H3 Ready-To-Use IHC Kit
Sample type FFPE tissue
Assay type Immunohistochemistry
Primary antibody type Rabbit Polyclonal
Secondary antibody type Polymer-HRP-Goat anti-Rabbit
Reactivity Human, Mouse, Rat

Kit components

ComponentSizeConcentration
Antigen Retrieval Buffer100 mL50×
Washing Buffer100 mL ×220×
Blocking Buffer5 mLRTU
Primary Antibody5 mLRTU
Secondary Antibody5 mLRTU
Chromogen Component A0.2 mLRTU
Chromogen Component B4 mLRTU
Signal Enhancer5 mLRTU
Counter Staining Reagent5 mLRTU
Mounting Media5 mLRTU
Datasheet1 Copy
Manual1 Copy

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

Properties

Storage Instructions All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt.
Synonyms H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone, histone 3, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o, Histone-H3