验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue, human placenta tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66171 targets Transferrin/TF in IF-P applications and shows reactivity with human samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | human |
| Immunogen | Transferrin/TF fusion protein Ag11668 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | transferrin |
| Synonyms | TF, transferrin, Serotransferrin, PRO2086, PRO1557 |
| Calculated Molecular Weight | 698 aa, 77 kDa |
| Observed Molecular Weight | 77 kDa |
| GenBank Accession Number | BC059367 |
| Gene Symbol | TF |
| Gene ID (NCBI) | 7018 |
| RRID | AB_2919293 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P02787 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
What is the tissue specificity of TF?
Transferrin is synthesized mainly in the liver.
What is the calculated molecular weight of TF?
77 kDa
How many iron atoms can bind to TF?
Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate
releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.
What is the primary role of TF?
TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.
What is the role of TF in human diseases?
TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a
decrease in hepcidin expression, causing increased iron levels.
Is TF essential for iron delivery?
Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while TF is not essential for iron delivery to all organs, it is needed for iron level regulation.
In what circumstances do TF levels decrease?
Inflammation and iron overload both can cause a decline in TF levels.
How does TF affect hormonal balance?
TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver.
How does TF deficiency cause increased iron levels?
TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF
deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues.
PMIDs: 23046645, 9635730, 22294463, 24589273
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Transferrin/TF antibody CL488-66171 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |