CoraLite® Plus 488-conjugated Transferrin/TF Monoclonal antibody

Transferrin/TF Monoclonal Antibody for IF-P
Cat No. CL488-66171

产品说明书

CloneNo. 2B4C6

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF-P

TF, transferrin, Serotransferrin, PRO2086, PRO1557

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive IF-P detected inhuman liver cancer tissue, human placenta tissue
Planning an IHC experiment? We recommend our IHCeasy TF Ready-To-Use IHC Kit. TF primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66171-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66171 targets Transferrin/TF in IF-P applications and shows reactivity with human samples.

经测试应用 IF-P Application Description
经测试反应性 human
免疫原 Transferrin/TF fusion protein Ag11668 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 transferrin
别名 TF, transferrin, Serotransferrin, PRO2086, PRO1557
计算分子量 698 aa, 77 kDa
观测分子量 77 kDa
GenBank蛋白编号BC059367
基因名称 TF
Gene ID (NCBI) 7018
RRIDAB_2919293
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP02787
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Transferrin/TF antibody CL488-66171Download protocol
Standard Protocols
Click here to view our Standard Protocols
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