|Positive WB detected in||human plasma, fetal human brain tissue|
|Positive IHC detected in||human liver cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human liver cancer tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:2000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66171-1-Ig targets Transferrin in WB, IHC, IF applications and shows reactivity with human samples.
|Tested Applications||IF, IHC, WB, ELISA|
|Cited Reactivity||human, rat|
|Immunogen||Transferrin fusion protein Ag11668|
|Host / Isotype||Mouse / IgG1|
|Synonyms||Beta 1 metal binding globulin, PRO1557, PRO2086, Serotransferrin, Siderophilin, TF, Transferrin|
|Calculated molecular weight||698 aa, 77 kDa|
|Observed molecular weight||77 kDa|
|GenBank accession number||BC059367|
|Gene ID (NCBI)||7018|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
What is the tissue specificity of TF?
Transferrin is synthesized mainly in the liver.
What is the calculated molecular weight of TF?
How many iron atoms can bind to TF?
Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate
releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.
What is the primary role of TF?
TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.
What is the role of TF in human diseases?
TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a
decrease in hepcidin expression, causing increased iron levels.
Is TF essential for iron delivery?
Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while TF is not essential for iron delivery to all organs, it is needed for iron level regulation.
In what circumstances do TF levels decrease?
Inflammation and iron overload both can cause a decline in TF levels.
How does TF affect hormonal balance?
TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver.
How does TF deficiency cause increased iron levels?
TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF
deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues.
PMIDs: 23046645, 9635730, 22294463, 24589273