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Transferrin/TF Monoclonal antibody, PBS Only

Transferrin/TF Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

2B4C6

Cat no : 66171-1-PBS

Print datasheet

Synonyms

TF, transferrin, Serotransferrin, PRO2086, PRO1557

验证数据展示

  • WB analysis of human plasma using 66171-1-Ig (same clone as 66171-1-PBS)

    WB analysis of human plasma using 66171-1-Ig (same clone as 66171-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66171-1-Ig (Transferrin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

    IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TF antibody included
  • IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

    IHC staining of human liver cancer using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66171-1-Ig (Transferrin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with TF antibody included
  • IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    IF Staining of human liver using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 66171-1-Ig (same clone as 66171-1-PBS)

    IF Staining of human placenta using 66171-1-Ig (same clone as 66171-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using Transferrin antibody (66171-1-Ig, Clone: 2B4C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66171-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy TF Ready-To-Use IHC Kit. TF primary antibody included.

产品信息

66171-1-PBS targets Transferrin/TF in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human
Immunogen Transferrin/TF fusion protein Ag11668 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name transferrin
Synonyms TF, transferrin, Serotransferrin, PRO2086, PRO1557
Calculated Molecular Weight 698 aa, 77 kDa
Observed Molecular Weight 77 kDa
GenBank Accession NumberBC059367
Gene Symbol TF
Gene ID (NCBI) 7018
RRIDAB_2881566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP02787
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

What is the tissue specificity of TF?

Transferrin is synthesized mainly in the liver.

What is the calculated molecular weight of TF?

77 kDa 

How many iron atoms can bind to TF?

Each of the two lobes of TF has one high-affinity Fe(III) binding site. Since binding of iron and bicarbonate 

releases free H+ ions, affinity decreases in acidic conditions, in which TF also remains bound to its receptor.

What is the primary role of TF?

TF serves as a serum metal-binding protein, playing a key role in iron metabolism and delivery for erythropoiesis.

What is the role of TF in human diseases?

TF deficiency results in an exhibition of congenital atransferrinemia (hypotransferrinemia) in which there is a 

decrease in hepcidin expression, causing increased iron levels. 

Is TF essential for iron delivery?

Congenital atransferrinemia results in an iron overload in some tissues, such as the liver, suggesting that while  TF is not essential for iron delivery to all organs, it is needed for iron level regulation.

In what circumstances do TF levels decrease?

Inflammation and iron overload both can cause a decline in TF levels.

How does TF affect hormonal balance?

TF positively regulates expression of hepcidin, a peptide hormone synthesized by the liver. 

How does TF deficiency cause increased iron levels? 

TF regulates hepcidin, which in turn is a negative regulator of intestinal iron absorption, resulting in TF 

deficiency causing both anemia, and, paradoxically, increased iron levels in some tissues. 

PMIDs: 23046645, 9635730, 22294463, 24589273