HRP-conjugated GFP tag Monoclonal antibody

GFP tag Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein, aequorea victoria and More (2)

Applications

WB and More (2)

Conjugate

HRP

CloneNo.

1E10H7

Cat No : HRP-66002

Print datasheet

Synonyms

CFP, eGFP, eYFP, GFP, GFP tag, GFP-tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP



经过测试的应用

Positive WB detected inRecombinant protein

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-66002 targets GFP tag in WB, IF, CoIP applications and shows reactivity with recombinant protein, aequorea victoria samples.

Tested Applications WB
Cited ApplicationsWB, IF, CoIP
Tested Reactivity recombinant protein, aequorea victoria
Cited Reactivityhuman, mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GFP tag
Synonyms CFP, eGFP, eYFP, GFP, GFP tag, GFP-tag, HRP CFP, HRP eGFP, HRP GFP, HRP YFP, YFP
Calculated Molecular Weight 26 kDa
Observed Molecular Weight-
GenBank Accession NumberU73901
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883834
Conjugate HRP
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a HRP conjugated mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.


Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.


发表文章

SpeciesApplicationTitle
WB

Cell

Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria.

Authors - Rui Yang
WB

Mol Neurodegener

Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease.

Authors - Guoxin Zhang

Elife

A picorna-like virus suppresses the N-end rule pathway to inhibit apoptosis.

Authors - Zhaowei Wang
WB

Elife

Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction.

Authors - Xingyu Zhang

Plant Physiol

Upstream Open Reading Frame and Phosphate-Regulated Expression of Rice OsNLA1 Controls Phosphate Transport and Reproduction.

Authors - Shu-Yi Yang
WB

Front Microbiol

The MERS-CoV N Protein Regulates Host Cytokinesis and Protein Translation via Interaction With EF1A.

Authors - Lin Zhu