GFP tag Monoclonal antibody
GFP tag Monoclonal Antibody for WB, IP, IF/ICC, FC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein and More (8)
Applications
WB, IP, IF/ICC, FC, ELISA and More (4)
Conjugate
Unconjugated
445
CloneNo.
1E10H7
验证数据展示
经过测试的应用
| Positive WB detected in | GFP transgenic mouse brain tissue, Recombinant protein |
| Positive IP detected in | Transfected HEK-293 cells |
| Positive IF detected in | Transfected HEK-293 cells |
| Positive FC detected in | Transfected HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF) | IF : 1:400-1:1600 |
| Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66002-1-Ig targets GFP tag in WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, IP, IF/ICC, FC, ELISA Application Description |
| Cited Applications | WB, IP, IF, FC, RIP, IHC, CoIP, ChIP, ELISA |
| Tested Reactivity | recombinant protein |
| Cited Reactivity | human, mouse, rat, monkey, zebrafish, hamster, pig, duck |
| Immunogen | GFP tag fusion protein Ag2128 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | GFP tag |
| Synonyms | CFP, eGFP, eYFP, GFP, GFP tag, GFP-tag, YFP |
| Calculated Molecular Weight | 26 kDa |
| GenBank Accession Number | U73901 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_11182611 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages.
Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for GFP tag antibody 66002-1-Ig | Download protocol |
| IF protocol for GFP tag antibody 66002-1-Ig | Download protocol |
| IP protocol for GFP tag antibody 66002-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Discov Glc7/PP1 dephosphorylates histone H3T11 to regulate autophagy and telomere silencing in response to nutrient availability | ||
Nat Genet Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome. | ||
Cell Stem Cell DUX-miR-344-ZMYM2-Mediated Activation of MERVL LTRs Induces a Totipotent 2C-like State. | ||
Nat Neurosci Reversal of prolonged obesity-associated cerebrovascular dysfunction by inhibiting microglial Tak1. | ||
Nat Biomed Eng Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids. |
