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验证数据展示

  • WB analysis using 66002-1-Ig (same clone as 66002-1-PBS)

    WB analysis using 66002-1-Ig (same clone as 66002-1-PBS)

    HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293T using 66002-1-Ig (same clone as 66002-1-PBS)

    IP experiment of Transfected HEK-293T using 66002-1-Ig (same clone as 66002-1-PBS)

    IP result of anti-GFP tag (IP:66002-1-Ig, 4ug; Detection:66002-1-Ig 1:10000) with Transfected HEK-293T cells lysate 400 ug. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    Immunofluorescent analysis of Transfected HEK-293 cells using 66002-1-Ig (GFP tag antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (66002-1-Ig, Clone: 1E10H7 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-3). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

Aequorea victoria GFP tag Monoclonal antibody, PBS Only

GFP tag Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 66002-1-PBS

产品说明书

CloneNo. 1E10H7

宿主/亚型

Mouse / IgG2a

种属反应性

recombinant protein

应用

WB, IF/ICC, IP, ELISA

别名

eGFP, GFP, CFP, eYFP, GFP-tag

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66002-1-PBS targets GFP tag in WB, IF/ICC, IP, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 recombinant protein
免疫原 GFP tag fusion protein Ag2128 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 GFP tag
别名 eGFP, GFP, CFP, eYFP, GFP-tag
计算分子量 26 kDa
GenBank蛋白编号M62653
基因名称
Gene ID (NCBI)
RRIDAB_11182611
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.