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Aequorea victoria GFP tag Monoclonal antibody, PBS Only

GFP tag Monoclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

recombinant protein

Applications

WB, IP, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1E10H7

Cat No : 66002-1-PBS

Print datasheet

Synonyms

CFP, eGFP, eYFP, GFP, YFP

验证数据展示

  • WB analysis using 66002-1-Ig (same clone as 66002-1-PBS)

    WB analysis using 66002-1-Ig (same clone as 66002-1-PBS)

    HEK293 cells, CHO cells, normal mouse brain and GFP transgenic mouse brain were subjected to SDS PAGE followed by western blot with 66002-1-Ig (GFP tag antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • IP experiment of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    IP experiment of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    IP result of anti-GFP tag (IP:66002-1-Ig, 4ug; Detection:66002-1-Ig 1:2000) with the vector plasmid pEGFP-N1 Transfected HEK-293 cells lysate 300ug. This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    Immunofluorescent analysis of Transfected HEK-293 cells using 66002-1-Ig (GFP tag antibody) at dilution of 1:50 and Alexa Fluor 594-conjugated AffiniPure Goat Anti-Mouse IgG (H+L). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

  • IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    IF Staining of Transfected HEK-293 using 66002-1-Ig (same clone as 66002-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using GFP tag antibody (66002-1-Ig, Clone: 1E10H7 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66002-1-PBS in a different storage buffer formulation.

产品信息

66002-1-PBS targets GFP tag in WB, IP, IF, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications WB, IP, IF, ELISA Application Description
Tested Reactivity recombinant protein
Immunogen GFP tag fusion protein Ag2128 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name GFP tag
Synonyms CFP, eGFP, eYFP, GFP, YFP
Calculated Molecular Weight 26 kDa
GenBank Accession NumberU73901
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages. 

Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.

When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.

This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.