CoraLite® Plus 488-conjugated GFP tag Monoclonal antibody
GFP tag Monoclonal Antibody for WB, IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
recombinant protein
Applications
WB, IF/ICC
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1E10H7
验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
| Positive IF/ICC detected in | Transfected HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66002 targets GFP tag in WB, IF/ICC applications and shows reactivity with recombinant protein samples.
| Tested Applications | WB, IF/ICC Application Description |
| Tested Reactivity | recombinant protein |
| Immunogen | GFP tag fusion protein Ag2128 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | GFP tag |
| Synonyms | eGFP, GFP, green, GFP-tag, eYFP |
| Calculated Molecular Weight | 26 kDa |
| GenBank Accession Number | M62653 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2919276 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages.
Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP. This antibody is labeled with CoraLite Plus 488.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 GFP tag antibody CL488-66002 | Download protocol |
| IF protocol for CL Plus 488 GFP tag antibody CL488-66002 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |