验证数据展示

WB analysis of HEK-293 using 55187-1-AP
WB result of ATP1A1-Specific antibody (55187-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1-Specific transfected HEK-293 cells.
WB analysis of mouse brain using 55187-1-AP
37°C incubated mouse brain membrane extract lysate and 37°C incubated mouse heart membrane extract lysate were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.
IF Staining of HEK-293 using 55187-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1-Specific antibody (55187-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of human liver cancer using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ATP1A1 antibody included
WB analysis using 55187-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IF Staining of Caco-2 using 55187-1-AP
Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IHC staining of mouse kidney using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ATP1A1 antibody included
IHC staining of human ovary cancer using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ATP1A1 antibody included
IHC staining of human liver cancer using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ATP1A1 antibody included
IHC staining of human colon using 55187-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Ready-to-use IHC kits available with ATP1A1 antibody included
FC experiment of HEK-293 using 55187-1-AP
1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1-Specific Polyclonal antibody (55187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

ATP1A1-Specific Polyclonal antibody

ATP1A1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 55187-1-AP

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Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Synonyms

ATP1A1, ATP1A 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (19)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	37°C incubated mouse brain tissue, HEK-293 cells, HepG2 cells, MCF-7 cells, 37°C incubated mouse heart tissue, Neuro-2a cells
Positive IHC detected in	human ovary cancer tissue, human colon tissue, human kidney tissue, human liver cancer tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HEK-293 cells, Caco-2 cells
Positive FC (Intra) detected in	HEK-293 cells

For optimal WB detection with 55187-1-AP, we do not recommend boiling the sample after lysis.

Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.

Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WB	See 13 publications below
IF	See 7 publications below

产品信息

55187-1-AP targets ATP1A1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited Applications	WB, IF
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Immunogen	Peptide 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms	ATP1A1, ATP1A 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13
Calculated Molecular Weight	113 kDa
Observed Molecular Weight	100-110 kDa
GenBank Accession Number	NM_000701
Gene Symbol	ATP1A1
Gene ID (NCBI)	476
ENSEMBL Gene ID	ENSG00000163399
RRID	AB_10859261
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P05023
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

实验方案

Product Specific Protocols
WB protocol for ATP1A1-Specific antibody 55187-1-AP	Download protocol
IHC protocol for ATP1A1-Specific antibody 55187-1-AP	Download protocol
IF protocol for ATP1A1-Specific antibody 55187-1-AP	Download protocol
FC protocol for ATP1A1-Specific antibody 55187-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
human	IF	Autophagy Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury Authors - Yanping Li View Article
mouse	WB	Hypertension Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension. Authors - Shuai Shao View Article
mouse	IF	Mol Biol Cell Retinoschisin is linked to retinal Na/K-ATPase signaling and localization. Authors - Karolina Plössl View Article
human	IF	Sci Rep Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification. Authors - Yan Xu View Article
human	WB	PLoS One A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice. Authors - Miao Liu View Article
human	WB	Exp Eye Res CHIR99021 balance TGFβ1 induced human corneal endothelial-to-mesenchymal transition to favor corneal endothelial cell proliferation. Authors - Yiran Wang View Article

验证数据展示

WB analysis of HEK-293 using 55187-1-AP

WB analysis of mouse brain using 55187-1-AP

IF Staining of HEK-293 using 55187-1-AP

IHC staining of human liver cancer using 55187-1-AP

WB analysis using 55187-1-AP

IF Staining of Caco-2 using 55187-1-AP

IHC staining of mouse kidney using 55187-1-AP

IHC staining of human ovary cancer using 55187-1-AP

IHC staining of human liver cancer using 55187-1-AP

IHC staining of human colon using 55187-1-AP

FC experiment of HEK-293 using 55187-1-AP

ATP1A1-Specific Polyclonal antibody

ATP1A1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

-/ -

经过测试的应用

For optimal WB detection with 55187-1-AP, we do not recommend boiling the sample after lysis.

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury

Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification.

A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice.

CHIR99021 balance TGFβ1 induced human corneal endothelial-to-mesenchymal transition to favor corneal endothelial cell proliferation.