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  • Featured Product
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ATP1A1-Specific Polyclonal antibody

ATP1A1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 55187-1-AP

Print datasheet

Synonyms

ATP1A1, ATP1A 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13

验证数据展示

  • WB analysis of HEK-293 using 55187-1-AP

    WB analysis of HEK-293 using 55187-1-AP

    WB result of ATP1A1-Specific antibody (55187-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATP1A1-Specific transfected HEK-293 cells.

  • WB analysis using 55187-1-AP

    WB analysis using 55187-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 55187-1-AP

    IF Staining of HEK-293 using 55187-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using ATP1A1-Specific antibody (55187-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of human liver cancer using 55187-1-AP

    IHC staining of human liver cancer using 55187-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IHC staining of human liver cancer using 55187-1-AP

    IHC staining of human liver cancer using 55187-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IF Staining of Caco-2 using 55187-1-AP

    IF Staining of Caco-2 using 55187-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IHC staining of mouse kidney using 55187-1-AP

    IHC staining of mouse kidney using 55187-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • IHC staining of human colon using 55187-1-AP

    IHC staining of human colon using 55187-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 55187-1-AP (ATP1A1-Specific antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ATP1A1 antibody included
  • FC experiment of HEK-293 using 55187-1-AP

    FC experiment of HEK-293 using 55187-1-AP

    1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug ATP1A1-Specific Polyclonal antibody (55187-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHEK-293 cells, HepG2 cells, mouse heart tissue, MCF-7 cells, Neuro-2a cells
Positive IHC detected inhuman liver cancer tissue, mouse kidney tissue, human kidney tissue, human normal colon
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells, Caco-2 cells
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55187-1-AP targets ATP1A1-Specific in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms ATP1A1, ATP1A 1, Na(+)/K(+) ATPase alpha-1 subunit, Na(+)/K(+) ATPase 1, EC:7.2.2.13
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberNM_000701
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
RRIDAB_10859261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05023
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

实验方案

Product Specific Protocols
WB protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
IHC protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
IF protocol for ATP1A1-Specific antibody 55187-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Autophagy

Hepatocyte CD36 modulates UBQLN1-mediated proteasomal degradation of autophagic SNARE proteins contributing to septic liver injury

Authors - Yanping Li
View Article
mouseWB

Hypertension

Renal Natriuretic Peptide Receptor-C Deficiency Attenuates NaCl Cotransporter Activity in Angiotensin II-Induced Hypertension.

Authors - Shuai Shao
View Article
mouseIF

Mol Biol Cell

Retinoschisin is linked to retinal Na/K-ATPase signaling and localization.

Authors - Karolina Plössl
View Article
humanIF

Sci Rep

Fluorescence- and magnetic-activated cell sorting strategies to separate spermatozoa involving plural contributors from biological mixtures for human identification.

Authors - Yan Xu
View Article
humanWB

PLoS One

A Novel Bufalin Derivative Exhibited Stronger Apoptosis-Inducing Effect than Bufalin in A549 Lung Cancer Cells and Lower Acute Toxicity in Mice.

Authors - Miao Liu
View Article
humanWB

Exp Eye Res

CHIR99021 balance TGFβ1 induced human corneal endothelial-to-mesenchymal transition to favor corneal endothelial cell proliferation.

Authors - Yiran Wang
View Article