验证数据展示
经过测试的应用
Positive IF/ICC detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55187-1-AP
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-55187 targets ATP1A1-Specific in IF/ICC applications and shows reactivity with human, mouse samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | human, mouse |
免疫原 | Peptide 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | ATPase, Na+/K+ transporting, alpha 1 polypeptide |
别名 | |
计算分子量 | 113 kDa |
观测分子量 | 100-110 kDa |
GenBank蛋白编号 | NM_000701 |
基因名称 | ATP1A1 |
Gene ID (NCBI) | 476 |
ENSEMBL Gene ID | ENSG00000163399 |
偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
最大激发/发射波长 | 654 nm / 674 nm |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P05023 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 ATP1A1-Specific antibody CL647-55187 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |