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验证数据展示

  • IF Staining of HEK-293 using CL647-55187

    IF Staining of HEK-293 using CL647-55187

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite® Plus 647 ATP1A1-Specific antibody (CL647-55187) at dilution of 1:200.

  • Featured Product
  • KD/KO Validated

CoraLite® Plus 647-conjugated ATP1A1-Specific Polyclonal antibody

ATP1A1-Specific Polyclonal Antibody for IF/ICC
Cat No. CL647-55187

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 647
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy ATP1A1 Ready-To-Use IHC Kit. ATP1A1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 55187-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-55187 targets ATP1A1-Specific in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ATPase, Na+/K+ transporting, alpha 1 polypeptide
Synonyms
Calculated Molecular Weight 113 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberNM_000701
Gene Symbol ATP1A1
Gene ID (NCBI) 476
ENSEMBL Gene IDENSG00000163399
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05023
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ATP1A1 is the catalytic component of Na+/K+-ATPase which is a membrane bound enzyme primarily involved in generation of Na+ and K+ gradients across plasma membranes and in determination of cytoplasmic Na+ levels. ATP1A1 is a ubiquitously expressed membrane protein and often used as the marker or internal control for plasma membrane protein. This antibody is specific to ATP1A1.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 ATP1A1-Specific antibody CL647-55187Download protocol
Standard Protocols
Click here to view our Standard Protocols