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验证数据展示

  • WB analysis using 66305-2-Ig

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66305-2-Ig

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis using 66305-2-Ig

    WB analysis using 66305-2-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 66305-2-Ig

    IHC staining of human ovary cancer using 66305-2-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of hTERT-RPE1 using 66305-2-Ig

    IF Staining of hTERT-RPE1 using 66305-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody (66305-2-Ig, Clone: 3A11F8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

Vinculin Monoclonal antibody

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66305-2-Ig

产品说明书

CloneNo. 3A11F8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, IF/ICC, ELISA

别名

VCL, MVCL, MV, Metavinculin, CMD1W

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHGC-27 cells, HUVEC cells, LNCaP cells, MKN-45 cells, K-562 cells, human peripheral blood platelets, rat heart tissue, mouse heart tissue, rabbit heart tissue, HeLa cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66305-2-Ig targets Vinculin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 Vinculin fusion protein Ag24946 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vinculin
别名 VCL, MVCL, MV, Metavinculin, CMD1W
计算分子量 1133 aa, 124 kDa
观测分子量124 kDa
GenBank蛋白编号BC039174
基因名称 Vinculin
Gene ID (NCBI) 7414
RRIDAB_3670375
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP18206
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

实验方案

Product Specific Protocols
WB protocol for Vinculin antibody 66305-2-IgDownload protocol
IHC protocol for Vinculin antibody 66305-2-IgDownload protocol
IF protocol for Vinculin antibody 66305-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols