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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    WB analysis using 66305-2-Ig (same clone as 66305-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66305-2-Ig (Vinculin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 66305-2-Ig (same clone as 66305-2-PBS)

    IHC staining of human ovary cancer using 66305-2-Ig (same clone as 66305-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 66305-2-Ig (Vinculin antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

  • IF Staining of hTERT-RPE1 using 66305-2-Ig (same clone as 66305-2-PBS)

    IF Staining of hTERT-RPE1 using 66305-2-Ig (same clone as 66305-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using Vinculin antibody (66305-2-Ig, Clone: 3A11F8 ) at dilution of 1:2000 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66305-2-PBS in a different storage buffer formulation.

Vinculin Monoclonal antibody, PBS Only

Vinculin Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 66305-2-PBS

产品说明书

CloneNo. 3A11F8

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, rabbit

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

VCL, MVCL, MV, Metavinculin, CMD1W

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66305-2-PBS targets Vinculin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, rabbit
免疫原 Vinculin fusion protein Ag24946 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 vinculin
别名 VCL, MVCL, MV, Metavinculin, CMD1W
计算分子量 1133 aa, 124 kDa
观测分子量124 kDa
GenBank蛋白编号BC039174
基因名称 Vinculin
Gene ID (NCBI) 7414
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP18206
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.