HRP-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, plant and More (6)

Applications

WB and More (3)

Conjugate

HRP Fluorescent Dye

CloneNo.

1E6D9

Cat No : HRP-60004

Print datasheet

Synonyms

G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

产品信息

HRP-60004 targets GAPDH in WB, IP, IHC, IF applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Applications WB
Cited Applications IF, IHC, IP, WB
Tested Reactivity human, mouse, rat, zebrafish, plant
Cited Reactivityhuman, mouse, rat, Chicken, hamster, Honey bees, opossum, pig, rabbit
Immunogen Recombinant Protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
UniprotP04406
Gene ID (NCBI) 2597
RRIDAB_2737588
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

实验方案

Product Specific Protocols
WB protocol for HRP GAPDH antibody HRP-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.

Authors - Linbin Yang
humanWB

Nat Biotechnol

Direct targeting of amplified gene loci for proapoptotic anticancer therapy.

Authors - Meetu Kaushik Tiwari
humanWB

Cell

Programmable RNA-Guided RNA Effector Proteins Built from Human Parts.

Authors - Simone Rauch
humanWB

Cell

Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages.

Authors - Shicheng Su
humanWB

Cell

CD10+GPR77+Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness.

Authors - Shicheng Su
human,mouseWB

Cell

PMID: 28753426

Authors - Kun Chen