HRP-conjugated GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, zebrafish, plant and More (6)

Applications

WB and More (2)

Conjugate

HRP Fluorescent Dye

CloneNo.

1E6D9

Cat No : HRP-60004

Print datasheet

Synonyms

G3PD, GAPD, GAPDH, HRP-G3PD, HRP-GAPD, HRP-GAPDH, HRP-OK/SW cl.12, OK/SW cl.12



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Sample-dependent, check data in validation data gallery

产品信息

HRP-60004 targets GAPDH in WB, IHC, IF applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.

Tested Applications WB
Cited Applications IF, IHC, WB
Tested Reactivity human, mouse, rat, zebrafish, plant
Cited Reactivityhuman, mouse, rat, Chicken, hamster, Honey bees, opossum, pig, rabbit
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant Protein
Full Name glyceraldehyde-3-phosphate dehydrogenase
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
UniprotP04406
Gene ID (NCBI) 2597
Conjugate HRP Fluorescent Dye
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

实验方案

Product Specific Protocols
WB protocol for HRP GAPDH antibody HRP-60004Download protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Sci Adv

MRGPR-mediated activation of local mast cells clears cutaneous bacterial infection and protects against reinfection.

Authors - Mohammad Arifuzzaman
humanWB

Cancers (Basel)

Transfection with GLS2 Glutaminase (GAB) Sensitizes Human Glioblastoma Cell Lines to Oxidative Stress by a Common Mechanism Involving Suppression of the PI3K/AKT Pathway.

Authors - Ewelina Majewska
mouseWB

Nature

Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells.

Authors - Dali Han
humanWB

Mol Med Rep

BP‑1‑102 exerts an antitumor effect on the AGS human gastric cancer cell line through modulating the STAT3 and MAPK signaling pathways.

Authors - Xiaoxia Jiang
humanWB

Hum Reprod

Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.

Authors - Yanling Gou
mouseWB

Int J Biol Sci

CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17.

Authors - Jie Liu