HRP-conjugated GAPDH Monoclonal antibody
GAPDH Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, zebrafish, plant and More (6)
Applications
WB and More (2)
Conjugate
HRP Fluorescent Dye
322
CloneNo.
1E6D9
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HepG2 cells, HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Sample-dependent, check data in validation data gallery | |
产品信息
HRP-60004 targets GAPDH in WB, IHC, IF applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.
| Tested Applications | WB |
| Cited Applications | IF, IHC, WB |
| Tested Reactivity | human, mouse, rat, zebrafish, plant |
| Cited Reactivity | human, mouse, rat, Chicken, hamster, Honey bees, opossum, pig, rabbit |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Recombinant Protein |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Calculated molecular weight | 36 kDa |
| Observed molecular weight | 36 kDa |
| GenBank accession number | BC004109 |
| Gene symbol | GAPDH |
| Uniprot | P04406 | Gene ID (NCBI) | 2597 |
| Conjugate | HRP Fluorescent Dye |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP GAPDH antibody HRP-60004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Sci Adv MRGPR-mediated activation of local mast cells clears cutaneous bacterial infection and protects against reinfection. | ||
Cancers (Basel) Transfection with GLS2 Glutaminase (GAB) Sensitizes Human Glioblastoma Cell Lines to Oxidative Stress by a Common Mechanism Involving Suppression of the PI3K/AKT Pathway. | ||
Nature Anti-tumour immunity controlled through mRNA m6A methylation and YTHDF1 in dendritic cells. | ||
Mol Med Rep BP‑1‑102 exerts an antitumor effect on the AGS human gastric cancer cell line through modulating the STAT3 and MAPK signaling pathways. | ||
Hum Reprod Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis. | ||
Int J Biol Sci CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17. |
