- Featured Product
- KD/KO Validated
HRP-conjugated GAPDH Monoclonal antibody
GAPDH Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat, zebrafish, plant and More (7)
Applications
WB and More (4)
Conjugate
HRP
691
CloneNo.
1E6D9
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, HepG2 cells, HEK-293 cells, mouse lung tissue, mouse liver tissue, mouse kidney tissue, mouse heart tissue, mouse thymus tissue, mouse spleen tissue. mouse skin tissue |
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
HRP-60004 targets GAPDH in WB, IF, IP, CoIP, RIP applications and shows reactivity with human, mouse, rat, zebrafish, plant samples.
| Tested Applications | WB Application Description |
| Cited Applications | WB, IF, IP, CoIP, RIP |
| Tested Reactivity | human, mouse, rat, zebrafish, plant |
| Cited Reactivity | human, mouse, rat, pig, rabbit, canine, chicken, hamster, opossum, honey bees |
| Immunogen | Recombinant protein 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms | 1E6D9 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004109 |
| Gene Symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_2737588 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P04406 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP GAPDH antibody HRP-60004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer | ||
Nat Biotechnol Direct targeting of amplified gene loci for proapoptotic anticancer therapy. | ||
Cell Immune Checkpoint Inhibition Overcomes ADCP-Induced Immunosuppression by Macrophages. | ||
Cell CD10+GPR77+Cancer-Associated Fibroblasts Promote Cancer Formation and Chemoresistance by Sustaining Cancer Stemness. |
