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GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA
Cat No. 60004-1-Ig

产品说明书

CloneNo. 1E6D9
引用文献(12535)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, zebrafish, yeast, plant and More (7)

应用

WB, IF/ICC, FC (Intra), IP, ELISA and More (3)

1E6D9

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells, PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue
Positive IP detected inHeLa Cells
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:50000-1:500000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

60004-1-Ig targets GAPDH in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish, yeast, plant samples.

经测试应用 WB, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP, ELISA
经测试反应性 human, mouse, rat, pig, zebrafish, yeast, plant
文献引用反应性human, rat, chicken, goat, yeast, deer, whiteflies, tick, treeshrew, cynomorium songaricum
免疫原 GAPDH fusion protein Ag0766 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glyceraldehyde-3-phosphate dehydrogenase
别名 1E6D9
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004109
基因名称 GAPDH
Gene ID (NCBI) 2597
RRIDAB_2107436
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04406
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.


实验方案

Product Specific Protocols
WB protocol for GAPDH antibody 60004-1-IgDownload protocol
IF protocol for GAPDH antibody 60004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Nature

A signalling pathway for transcriptional regulation of sleep amount in mice

Authors - Rui Zhou
mouseWB

Cell Res

Tau is a receptor with low affinity for glucocorticoids and is required for glucocorticoid-induced bone loss

Authors - Wenyu Fu
mouse,humanWB

Cell Res

Nonenzymatic lysine D-lactylation induced by glyoxalase II substrate SLG dampens inflammatory immune responses

Authors - Qihang Zhao
humanWB

Nature

Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.

Authors - Zhaoyang Li
humanWB

Nature

Structure of LRRK2 in Parkinson's disease and model for microtubule interaction.

Authors - C K Deniston
humanWB

Nature

The molecular basis of tight nuclear tethering and inactivation of cGAS.

Authors - Baoyu Zhao
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