GAPDH Monoclonal antibody

GAPDH Monoclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, yeast, plant, zebrafish and More (10)

Applications

FC, IF, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

CloneNo.

1E6D9

Cat No : 60004-1-Ig

Print datasheet

Synonyms

G3PD, GAPD, GAPDH, OK/SW cl.12



经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, ROS1728 cells, pig brain tissue, zebrafish tissue, whole yeast, whole Nematode tissue, soybean whole plant tissue, arabidopsis whole plant tissue, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells, C6 cells, PC-12 cells, C2C12 cells, SP2/0 cells, rat brain tissue, mouse brain tissue
Positive IP detected inHeLa Cells
Positive IF detected inEthacrynic acid treated HeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®488, CoraLite®594 and CoraLite®650 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:50000-1:500000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:2000-1:12000 for WB
Immunofluorescence (IF)IF : 1:200-1:2000
Sample-dependent, check data in validation data gallery

产品信息

60004-1-Ig targets GAPDH in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, yeast, plant, zebrafish samples.

Tested Applications FC, IF, IP, WB, ELISA
Cited Applications ChIP, CoIP, FC, IF, IHC, IP, WB
Tested Reactivity human, mouse, rat, yeast, plant, zebrafish
Cited ReactivityArabidopsis, Bombyx mori, bovine, Branchiostoma belcheri, brovine, Caenorhabditis elegans, canine, chicken, CHO cell line, Cynomorium songaricum
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen GAPDH fusion protein Ag0766 种属同源性预测
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms G3PD, GAPD, GAPDH, OK/SW cl.12
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC004109
Gene symbol GAPDH
UniprotP04406
Gene ID (NCBI) 2597
RRIDAB_2107436
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.


实验方案

Product Specific Protocols
WB protocol for GAPDH antibody 60004-1-IgDownload protocol
IF protocol for GAPDH antibody 60004-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

Allele-selective lowering of mutant HTT protein by HTT-LC3 linker compounds.

Authors - Zhaoyang Li
humanWB

Nature

Structure of LRRK2 in Parkinson's disease and model for microtubule interaction.

Authors - C K Deniston
humanWB

Nature

The molecular basis of tight nuclear tethering and inactivation of cGAS.

Authors - Baoyu Zhao
human,mouseWB

Nature

Inhibition of PCSK9 potentiates immune checkpoint therapy for cancer.

Authors - Xinjian Liu
WB

Nature

A phase-separated nuclear GBPL circuit controls immunity in plants.

Authors - Shuai Huang
humanWB

Nature

SARS-CoV-2 B.1.617.2 Delta variant replication and immune evasion.

Authors - Petra Mlcochova