验证数据展示
经过测试的应用
| Positive WB detected in | |
| Positive IHC detected in | Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | human PBMCs |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Flow Cytometry (FC) | FC : 0.50 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
| ChIP | See 2 publications below |
产品信息
66360-3-Ig targets Mouse IgG2b isotype control in WB, IHC, FC, chIP, ELISA applications and shows reactivity with megathura crenulata samples.
| 经测试应用 | WB, IHC, FC, ELISA Application Description |
| 文献引用应用 | WB, chIP |
| 经测试反应性 | megathura crenulata |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Mouse IgG2b isotype control |
| 别名 | Mouse IgG2b |
| 基因名称 | |
| Gene ID (NCBI) | |
| RRID | AB_2881740 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| IHC protocol for Mouse IgG2b isotype control antibody 66360-3-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol The COX10-AS1/miR-641/E2F6 Feedback Loop Is Involved in the Progression of Glioma. | ||
Cell Death Dis E2F1 mediated DDX11 transcriptional activation promotes hepatocellular carcinoma progression through PI3K/AKT/mTOR pathway. | ||
Aging Dis Age-related Loss of miR-124 Causes Cognitive Deficits via Derepressing RyR3 Expression | ||
Immunity Ribosome-rescuer PELO catalyzes the oligomeric assembly of NOD-like receptor family proteins via activating their ATPase enzymatic activity | ||
Nat Commun Generation of nanobodies from transgenic 'LamaMice' lacking an endogenous immunoglobulin repertoire | ||
J Virol The neonatal Fc receptor (FcRn) is required for porcine reproductive and respiratory syndrome virus uncoating |