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CoraLite® Plus 647 Mouse IgG2b isotype control (11B8C4)

IgG2b isotype control Monoclonal Antibody for FC

Host / Isotype

Mouse / IgG2b

Reactivity

Megathura crenulata

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

11B8C4

Cat no : CL647-66360-3

Print datasheet

Synonyms

Mouse IgG2b, Mouse IgG2b isotype control

验证数据展示

  • FC experiment of HeLa using CL647-66360-3

    FC experiment of HeLa using CL647-66360-3

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Mouse IgG2b isotype control (CL647-66360-3, Clone:11B8C4) (red), or 0.4 ug CoraLite® Plus 647-conjugated Alpha Tubulin Monoclonal antibody (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected in
Positive FC detected in
For other applications, we recommend the unconjugated version of this antibody, 66360-3-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-66360-3 targets IgG2b isotype control in FC applications and shows reactivity with Megathura crenulata samples.

Tested Applications FC Application Description
Tested Reactivity Megathura crenulata
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name IgG2b isotype control
Synonyms Mouse IgG2b, Mouse IgG2b isotype control
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883735
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

0.05-0.2 ug per test is suggested for FC experiment.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 IgG2b isotype control antibody CL647-66360-3Download protocol
Standard Protocols
Click here to view our Standard Protocols