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Mouse IgG2b isotype control Monoclonal antibody, PBS Only

Mouse IgG2b isotype control Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

megathura crenulata

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

11B8C4

Cat no : 66360-3-PBS

Print datasheet

Synonyms

Mouse IgG2b

验证数据展示

  • WB analysis using 66360-3-Ig (same clone as 66360-3-PBS)

    WB analysis using 66360-3-Ig (same clone as 66360-3-PBS)

    Various lysates (50 ug/lane) were subjected to SDS PAGE followed by western blot with 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

    IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

    IHC staining of human colon using 66360-3-Ig (same clone as 66360-3-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66360-3-Ig (Mouse IgG2b isotype control antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

  • FC experiment of human PBMCs using 66360-3-Ig (same clone as 66360-3-PBS)

    FC experiment of human PBMCs using 66360-3-Ig (same clone as 66360-3-PBS)

    1x10^6 human PBMCs were surface stained with 0.5 ug Mouse IgG2b isotype control Monoclonal antibody (66360-3-Ig, Clone: 11B8C4) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.5 ug Anti-Human CD20 (65085-1-Ig) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (green), or unstained (blue). Cells were not fixed. This data was developed using the same antibody clone with 66360-3-PBS in a different storage buffer formulation.

产品信息

66360-3-PBS targets Mouse IgG2b isotype control in WB, IHC, ELISA applications and shows reactivity with megathura crenulata samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity megathura crenulata
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name Mouse IgG2b isotype control
Synonyms Mouse IgG2b
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry, Immuno-Precipitation and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).