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验证数据展示

  • WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis of HepG2 using 60004-1-Ig (same clone as 60004-1-PBS)

    Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa Cells using 60004-1-Ig (same clone as 60004-1-PBS)

    IP experiment of HeLa Cells using 60004-1-Ig (same clone as 60004-1-PBS)

    IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • WB analysis of arabidopsis whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis of arabidopsis whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

    arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 60004-1-Ig (same clone as 60004-1-PBS)

    IHC staining of human liver cancer using 60004-1-Ig (same clone as 60004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60004-1-Ig (GAPDH antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAPDH antibody included
  • IHC staining of human liver cancer using 60004-1-Ig (same clone as 60004-1-PBS)

    IHC staining of human liver cancer using 60004-1-Ig (same clone as 60004-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 60004-1-Ig (GAPDH antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GAPDH antibody included
  • IF Staining of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    IF Staining of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH antibody (60004-1-Ig, Clone: 1E6D9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    FC experiment of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GAPDH (60004-1-Ig, Clone:1E6D9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

GAPDH Monoclonal antibody, PBS Only

GAPDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 60004-1-PBS

产品说明书

CloneNo. 1E6D9
引用文献(2)

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig, zebrafish, yeast, plant and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

别名

1E6D9

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

产品信息

60004-1-PBS targets GAPDH in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish, yeast, plant samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat, pig, zebrafish, yeast, plant
文献引用反应性human, mouse, rabbit
免疫原 GAPDH fusion protein Ag0766 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 glyceraldehyde-3-phosphate dehydrogenase
别名 1E6D9
计算分子量 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC004109
基因名称 GAPDH
Gene ID (NCBI) 2597
RRIDAB_2107436
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP04406
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

What is the molecular weight of GAPDH?

The molecular weight of GAPDH is 36 kD.


What is GAPDH?

GAPDH stands for glyceraldehyde-3-phosphate dehydrogenase and is often referred to as a "housekeeping" 

protein. It is recognized as an important enzyme involved in metabolic pathways, aiding in the glycolytic 

production of ATP and pyruvate (PMID: 21895736).


What is the function of GAPDH?

GAPDH is responsible for catalyzing the phosphorylation of glyceraldehyde-3-phosphate into D-glycerate-1,3-biphosphate. While this function of GAPDH is considered its primary function, GAPDH is also involved, even 

critical, in other cell functions, some of which are still being studied. Recent studies have suggested that GAPDH 

is a key factor in regulating apoptosis, translocating to the nucleus under certain stressful conditions and acting 

as a signaling factor in oxidative stress-induced apoptosis. Other studies indicate that GAPDH plays a role in 

transporting vesicles from the endoplasmic reticulum to the Golgi body (PMIDs: 21895736, 12829261, 25859407, 

11035021).


Where in the cell is GAPDH localized?

GAPDH is expressed primarily in the cytosolic and membrane regions, with minimal expression in the nucleus 

(PMIDs: 21895736, 12829261).


Is there a reason to use an antibody targeting GAPDH if my research is not concerned with glycolysis or nuclear 

functions?

Yes! Because GAPDH is often highly expressed throughout different tissues and cell types, GAPDH expression is 

useful as a control for Western blots and RT-PCR.


What are the known protein-protein interactions of GAPDH?

GAPDH interacts with other proteins to regulate nuclear translocation and GAPDH catalytic activity. Some of 

these proteins include TPPP, SIAH1, CHP1, RILPL1/GOSPEL and FKBP6 (PMIDs: 21895736, 25859407).


Note: For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.


发表文章

SpeciesApplicationTitle
WB

Mol Cell Biochem

PSAT1 promotes the progression of colorectal cancer by regulating Hippo-YAP/TAZ-ID1 axis via AMOT

Authors - Minshan Tang
View Article
rabbit,mouse,humanWB,IP,IHC,IF,CoIP

Cancer Res

Tobacco Smoking Rewires Cell Metabolism by Inducing GAPDH Succinylation to Promote Lung Cancer Progression

Authors - Kun Wang
View Article