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  • Featured Product
  • KD/KO Validated

GAPDH Monoclonal antibody, PBS Only

GAPDH Monoclonal Antibody for WB, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig, zebrafish, yeast, plant

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

CloneNo.

1E6D9

Cat no : 60004-1-PBS

Print datasheet

Synonyms

1E6D9

验证数据展示

  • WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:200000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis of HepG2 using 60004-1-Ig (same clone as 60004-1-PBS)

    Western blot analysis of GAPDH in various tissues and cell lines using Proteintech antibody 60004-1-Ig at a dilution of 1:10000. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis using 60004-1-Ig (same clone as 60004-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa Cells using 60004-1-Ig (same clone as 60004-1-PBS)

    IP experiment of HeLa Cells using 60004-1-Ig (same clone as 60004-1-PBS)

    IP result of anti-GAPDH (60004-1-Ig for IP and Detection) with HeLa cell lysate. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • WB analysis of arabidopsis whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

    WB analysis of arabidopsis whole plant using 60004-1-Ig (same clone as 60004-1-PBS)

    arabidopsis, rice, wheat, corn whole plant tissue were subjected to SDS PAGE followed by western blot with 60004-1-Ig (GAPDH Antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    IF Staining of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using GAPDH antibody (60004-1-Ig, Clone: 1E6D9 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    FC experiment of HeLa using 60004-1-Ig (same clone as 60004-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human GAPDH (60004-1-Ig, Clone:1E6D9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 60004-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy GAPDH Ready-To-Use IHC Kit. GAPDH primary antibody included.

产品信息

60004-1-PBS targets GAPDH in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, pig, zebrafish, yeast, plant samples.

Tested Applications WB, IF/ICC, FC (Intra), IP, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, zebrafish, yeast, plant
Immunogen GAPDH fusion protein Ag0766 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name glyceraldehyde-3-phosphate dehydrogenase
Synonyms 1E6D9
Calculated Molecular Weight 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC004109
Gene Symbol GAPDH
Gene ID (NCBI) 2597
RRIDAB_2107436
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04406
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types. For murine tissue samples, conjugated mouse antibody HRP-60004 and rabbit antibody 10494-1-AP are preferable.