验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, HEK-293 cells, Jurkat cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
产品信息
CL488-60004 targets GAPDH in WB, FC (Intra) applications and shows reactivity with human, mouse, rat, zebrafish, yeast, plant samples.
| Tested Applications | WB, FC (Intra) Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat, zebrafish, yeast, plant |
| Cited Reactivity | human, mouse, rat |
| Immunogen | GAPDH fusion protein Ag0766 种属同源性预测 |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glyceraldehyde-3-phosphate dehydrogenase |
| Synonyms | glyceraldehyde 3 phosphate dehydrogenase, GAPD, EC:2.6.99.-, EC:1.2.1.12, CDABP0047 |
| Calculated Molecular Weight | 36 kDa |
| Observed Molecular Weight | 36 kDa |
| GenBank Accession Number | BC004109 |
| Gene Symbol | GAPDH |
| Gene ID (NCBI) | 2597 |
| RRID | AB_2919223 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04406 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. GAPDH participates in nuclear events including transcription, binding RNA, RNA transportation, DNA replication, DNA repair and apoptosis. Being stably and constitutively expressed at high levels in most tissues and cells, GAPDH is considered a housekeeping protein. It is widely used as a control for RT-PCR and also loading control in electrophoresis and Western blotting. GAPDH is normally expressed in cellular cytoplasm or membrane, but can occasionally translocate to the nucleus after the addition of post-translational modifications such as S-nitrosylation. This antibody is raised against full length GAPDH of human origin. It can recognize the 36 kDa GAPDH protein in most cells/tissues. In addition, a band below 36 kDa can always be detected as the isoform or spliced product of GAPDH (PMID: 23885286, 23877755, 19368702). Please note that some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 GAPDH antibody CL488-60004 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Aging (Albany NY) Coagulation factor 2 thrombin receptor promotes malignancy in glioma under SOX2 regulation. | ||
Cell Commun Signal Integral membrane protein 2A inhibits cell growth in human breast cancer via enhancing autophagy induction. | ||
Sci Rep Comparative analysis of pathophysiological parameters between emphysematous smokers and emphysematous patients with COPD. | ||
Exp Cell Res CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway. | ||
Virus Res Interaction of porcine reproductive and respiratory syndrome virus major envelope proteins GP5 and M with the cellular protein Snapin. | ||
Reprod Fertil Dev Effects of 2,3',4,4',5-pentachlorobiphenyl exposure during pregnancy on DNA methylation in the testis of offspring in the mouse. |