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CoraLite®488-conjugated YY1 Monoclonal antibody
YY1 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, monkey
Applications
IF/ICC
Conjugate
CoraLite®488 Fluorescent Dye
CloneNo.
2E11C5
验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66281-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66281 targets YY1 in IF/ICC applications and shows reactivity with human, mouse, rat, monkey samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat, monkey |
Immunogen | YY1 fusion protein Ag17732 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | YY1 transcription factor |
Synonyms | INO80S, UCRBP, YY1 |
Calculated Molecular Weight | 414 aa, 45 kDa |
Observed Molecular Weight | 65-70 kDa |
GenBank Accession Number | BC037308 |
Gene Symbol | YY1 |
Gene ID (NCBI) | 7528 |
RRID | AB_2883302 |
Conjugate | CoraLite®488 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 491 nm / 516 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P25490 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
实验方案
Product Specific Protocols | |
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IF protocol for CL488 YY1 antibody CL488-66281 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |