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CoraLite®488-conjugated YY1 Monoclonal antibody

YY1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey

Applications

IF/ICC

Conjugate

CoraLite®488 Fluorescent Dye

CloneNo.

2E11C5

Cat no : CL488-66281

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Synonyms

INO80S, UCRBP, YY1



经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66281-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66281 targets YY1 in IF/ICC applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat, monkey
Immunogen YY1 fusion protein Ag17732 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name YY1 transcription factor
Synonyms INO80S, UCRBP, YY1
Calculated Molecular Weight 414 aa, 45 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC037308
Gene Symbol YY1
Gene ID (NCBI) 7528
RRIDAB_2883302
Conjugate CoraLite®488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths491 nm / 516 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25490
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

实验方案

Product Specific Protocols
IF protocol for CL488 YY1 antibody CL488-66281Download protocol
Standard Protocols
Click here to view our Standard Protocols