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CoraLite® Plus 750-conjugated YY1 Monoclonal antibody

YY1 Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2E11C5

Cat No : CL750-66281

Print datasheet

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

验证数据展示

  • WB analysis using CL750-66281

    WB analysis using CL750-66281

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-66281 (YY1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inNIH/3T3 cells, HepG2 cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66281-1-Ig

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66281 targets YY1 in WB applications and shows reactivity with human, mouse samples.

Tested Applications WB
Tested Reactivity human, mouse
Immunogen YY1 fusion protein Ag17732 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name YY1 transcription factor
Synonyms DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor
Calculated Molecular Weight 414 aa, 45 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC037308
Gene Symbol YY1
Gene ID (NCBI) 7528
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25490
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 YY1 antibody CL750-66281Download protocol
Standard Protocols
Click here to view our Standard Protocols