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CoraLite® Plus 750-conjugated YY1 Monoclonal antibody

YY1 Monoclonal Antibody for WB
Cat No. CL750-66281

产品说明书

CloneNo. 2E11C5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -


经过测试的应用

Positive WB detected inNIH/3T3 cells, HepG2 cells, HUVEC cells
Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66281-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-66281 targets YY1 in WB applications and shows reactivity with human, mouse samples.

经测试应用 WB Application Description
经测试反应性 human, mouse
免疫原 YY1 fusion protein Ag17732 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 YY1 transcription factor
别名 DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor
计算分子量 414 aa, 45 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC037308
基因名称 YY1
Gene ID (NCBI) 7528
RRIDAB_3086571
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25490
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 YY1 antibody CL750-66281Download protocol
Standard Protocols
Click here to view our Standard Protocols
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