|Positive WB detected in||C6 cells, A431 cells, MCF-7 cells, NIH/3T3 cells, HeLa cells, COS-7 cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human breast cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66281-1-Ig targets YY1 in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.
|Tested Applications||IF, IHC, IP, WB, ELISA|
|Cited Applications||ChIP, CoIP, IF, IHC, WB|
|Tested Reactivity||human, mouse, rat, monkey|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||YY1 fusion protein Ag17732|
|Full Name||YY1 transcription factor|
|Calculated molecular weight||414 aa, 45 kDa|
|Observed molecular weight||65-70 kDa|
|GenBank accession number||BC037308|
|Uniprot||P25490||Gene ID (NCBI)||7528|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.
|Product Specific Protocols|
|WB protocol for YY1 antibody 66281-1-Ig||Download protocol|
|IHC protocol for YY1 antibody 66281-1-Ig||Download protocol|
|IF protocol for YY1 antibody 66281-1-Ig||Download protocol|
|IP protocol for YY1 antibody 66281-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
The p300/YY1/miR-500a-5p/HDAC2 signalling axis regulates cell proliferation in human colorectal cancer.
CircPSMC3 suppresses the proliferation and metastasis of gastric cancer by acting as a competitive endogenous RNA through sponging miR-296-5p.
J Cell Mol Med
FAM3C-YY1 axis is essential for TGFβ-promoted proliferation and migration of human breast cancer MDA-MB-231 cells via the activation of HSF1.
RNF144A exerts tumor suppressor function in breast cancer through targeting YY1 for proteasomal degradation to downregulate GMFG expression.
hnRNPM, a potential mediator of YY1 in promoting the epithelial-mesenchymal transition of prostate cancer cells.
Transcription factor YY1 enhances the stemness of lung cancer cells by stabilizing hypoxia factor HIF-1α under a hypoxic microenvironment.