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  • Featured Product
  • KD/KO Validated

Alpha Tubulin Recombinant antibody

Alpha Tubulin Recombinant Antibody for WB, IF/ICC,IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IF/ICC,IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1G7

Cat No : 80762-1-RR

Print datasheet

Synonyms

alpha tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, tubulin alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b

验证数据展示

  • IF Staining of C2C12 using 80762-1-RR

    IF Staining of C2C12 using 80762-1-RR

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • WB analysis using 80762-1-RR

    WB analysis using 80762-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80762-1-RR (Tubulin-Alpha antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HEK-293 using 80762-1-RR

    WB analysis of HEK-293 using 80762-1-RR

    WB result of Alpha Tubulin antibody (80762-1-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Alpha Tubulin transfected HEK-293 cells.

  • IF Staining of mouse testis using 80762-1-RR

    IF Staining of mouse testis using 80762-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using Alpha Tubulin antibody (80762-1-RR, Clone: 1G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, rat brain, mouse brain
Positive IF detected inC2C12 cells, mouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy TUBA1B Ready-To-Use IHC Kit. TUBA1B primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80762-1-RR targets Alpha Tubulin as part of a matched antibody pair:

MP00551-1: 80740-1-RR capture and 80762-1-RR detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF/ICC,IF-P, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Alpha Tubulin fusion protein Ag18034 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name tubulin, alpha 1b
Synonyms alpha tubulin, Alpha tubulin ubiquitous, alpha tubulin,a tubulin, K ALPHA 1, TUBA1B, tubulin alpha, Tubulin alpha 1B chain, Tubulin alpha ubiquitous chain, Tubulin K alpha 1, tubulin, alpha 1b
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC009314
Gene Symbol TUBA1B
Gene ID (NCBI) 10376
RRIDAB_2918911
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68363
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

There are five tubulins in human cells: alpha, beta, gamma, delta, and epsilon. Tubulins are conserved across species. They form heterodimers, which multimerize to form a microtubule filament. An alpha and beta tubulin heterodimer is the basic structural unit of microtubules. The heterodimer does not come apart once formed. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha tubulin is useful for scientists across fields as an internal control due to its high, ubiquitous expression pattern.

实验方案

Product Specific Protocols
WB protocol for Alpha Tubulin antibody 80762-1-RRDownload protocol
IF protocol for Alpha Tubulin antibody 80762-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Nat Biotechnol

Magnify is a universal molecular anchoring strategy for expansion microscopy

Authors - Aleksandra Klimas
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mouseWB

Dose Response

The Protective Effects of Apigenin Against Radiation-Induced Intestinal Injury.

Authors - Danjie Liu
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mouseWB

Mol Med Rep

Saikosaponin A protects against uremic toxin indole‑3 acetic acid‑induced damage to the myocardium

Authors - Cheng Chen
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humanWB

Mol Med

Liraglutide attenuates type 2 diabetes mellitus-associated non-alcoholic fatty liver disease by activating AMPK/ACC signaling and inhibiting ferroptosis

Authors - Tingli Guo
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