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验证数据展示

  • IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66281-1-Ig (YY1 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66281-1-Ig (same clone as 66281-1-PBS)

    WB analysis of HeLa using 66281-1-Ig (same clone as 66281-1-PBS)

    Western blot of YY1 in various human cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • WB analysis of C6 using 66281-1-Ig (same clone as 66281-1-PBS)

    WB analysis of C6 using 66281-1-Ig (same clone as 66281-1-PBS)

    Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 66281-1-Ig (same clone as 66281-1-PBS)

    IP experiment of NIH/3T3 using 66281-1-Ig (same clone as 66281-1-PBS)

    IP result of anti-YY1 (IP:66281-1-Ig, 5ug; Detection:66281-1-Ig 1:1000) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66281-1-Ig (same clone as 66281-1-PBS)

    WB analysis of MCF-7 using 66281-1-Ig (same clone as 66281-1-PBS)

    WB result of YY1 antibody (66281-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YY1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • WB analysis using 66281-1-Ig (same clone as 66281-1-PBS)

    WB analysis using 66281-1-Ig (same clone as 66281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IHC staining of human colon cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human colon cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (66281-1-Ig, Clone: 2E11C5 ) at dilution of 1:1800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

YY1 Monoclonal antibody, PBS Only

YY1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66281-1-PBS

产品说明书

CloneNo. 2E11C5

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat, monkey

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.

产品信息

66281-1-PBS targets YY1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, monkey
免疫原 YY1 fusion protein Ag17732 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 YY1 transcription factor
别名 DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor
计算分子量 414 aa, 45 kDa
观测分子量 65-70 kDa
GenBank蛋白编号BC037308
基因名称 YY1
Gene ID (NCBI) 7528
RRIDAB_2881664
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP25490
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.