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Species Reactivity
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验证数据展示

  • IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 66281-1-Ig (YY1 antibody), at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66281-1-Ig (same clone as 66281-1-PBS)

    WB analysis of HeLa using 66281-1-Ig (same clone as 66281-1-PBS)

    Western blot of YY1 in various human cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • WB analysis of C6 using 66281-1-Ig (same clone as 66281-1-PBS)

    WB analysis of C6 using 66281-1-Ig (same clone as 66281-1-PBS)

    Western blot of YY1 in rat (C6 and ROS1728) and mouse(NIH3T3 and Raw264.7) cell lines with 66281-1-Ig at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 66281-1-Ig (same clone as 66281-1-PBS)

    IP experiment of NIH/3T3 using 66281-1-Ig (same clone as 66281-1-PBS)

    IP result of anti-YY1 (IP:66281-1-Ig, 5ug; Detection:66281-1-Ig 1:1000) with NIH/3T3 cells lysate 4000ug. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 66281-1-Ig (same clone as 66281-1-PBS)

    WB analysis of MCF-7 using 66281-1-Ig (same clone as 66281-1-PBS)

    WB result of YY1 antibody (66281-1-Ig; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YY1 transfected MCF-7 cells. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • WB analysis using 66281-1-Ig (same clone as 66281-1-PBS)

    WB analysis using 66281-1-Ig (same clone as 66281-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66281-1-Ig (YY1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human breast cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IHC staining of human colon cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human colon cancer using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    IHC staining of human tonsillitis using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66281-1-Ig (YY1 antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YY1 antibody included
  • IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    IF Staining of HepG2 using 66281-1-Ig (same clone as 66281-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (66281-1-Ig, Clone: 2E11C5 ) at dilution of 1:1800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), Alpha Tubulin antibody (80762-1-RR, Clone: 1G7, red). This data was developed using the same antibody clone with 66281-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

YY1 Monoclonal antibody, PBS Only

YY1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 66281-1-PBS

Print datasheet

CloneNo. 2E11C5

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, monkey

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.

产品信息

66281-1-PBS targets YY1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, monkey
Immunogen YY1 fusion protein Ag17732 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name YY1 transcription factor
Synonyms DELTA, Delta transcription factor, INO80 complex subunit S, INO80S, NF E1, UCRBP, Yin and yang 1, YIN YANG 1, YY 1, YY1, YY1 transcription factor
Calculated Molecular Weight 414 aa, 45 kDa
Observed Molecular Weight 65-70 kDa
GenBank Accession NumberBC037308
Gene Symbol YY1
Gene ID (NCBI) 7528
RRIDAB_2881664
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP25490
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.