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CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

recombinant protein

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E7B4

Cat no : CL488-66008

Print datasheet

Synonyms

Flag tag, Flag, DYKDDDDK tag, DDDDK tag

验证数据展示

  • IF Staining of Transfected HEK-293 using CL488-66008

    IF Staining of Transfected HEK-293 using CL488-66008

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66008-3 (DDDDK tag antibody), at dilution of 1:100.

  • FC experiment of Transfected HEK-293T using CL488-66008

    FC experiment of Transfected HEK-293T using CL488-66008

    1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope) (CL488-66008, Clone:1E7B4)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66008 targets DYKDDDDK in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity recombinant protein
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name DYKDDDDK
Synonyms Flag tag, Flag, DYKDDDDK tag, DDDDK tag
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883224
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DYKDDDDK antibody CL488-66008Download protocol
Standard Protocols
Click here to view our Standard Protocols