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验证数据展示

  • IF Staining of Transfected HEK-293 using CL488-66008

    IF Staining of Transfected HEK-293 using CL488-66008

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using the CoraLite® Plus 488-conjugated version of this antibody, CL488-66008-3 (DDDDK tag antibody), at dilution of 1:100.

  • FC experiment of Transfected HEK-293T using CL488-66008

    FC experiment of Transfected HEK-293T using CL488-66008

    1x10^6 Transfected HEK-293T cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope) (CL488-66008, Clone:1E7B4)(red). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated DYKDDDDK Monoclonal antibody (Binds to FLAG® tag epitope)

DYKDDDDK Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66008

产品说明书

CloneNo. 1E7B4

宿主/亚型

Mouse / IgG2b

种属反应性

recombinant protein

应用

IF/ICC, FC (Intra)

别名

Flag tag, Flag, DYKDDDDK tag, DDDDK tag

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells
Positive FC (Intra) detected inTransfected HEK-293T cells

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66008 targets DYKDDDDK in IF/ICC, FC (Intra) applications and shows reactivity with recombinant protein samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 recombinant protein
免疫原 Peptide 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 Flag Tag
别名 Flag tag, Flag, DYKDDDDK tag, DDDDK tag
基因名称 Flag Tag
Gene ID (NCBI) 8
RRIDAB_2883224
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYKDDDDK Tag ( Equivalent To FLAG Antibody From Sigma ) with the following sequence DYKDDDDK, is a hydrophilic tag for recombinant protein technology. Tags can be used as a tool to localize gene products in a variety of cell types, study proteins topology, and also help to identify and characterize new, low abundance or poorly immunogenic proteins. Due to its high hydrophilic character, the DDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag to be accessible for antibodies. DDDDK Tag Antibody is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DYKDDDDK antibody CL488-66008Download protocol
Standard Protocols
Click here to view our Standard Protocols