验证数据展示
经过测试的应用
| Positive WB detected in | Recombinant protein |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:100000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 21 publications below |
| ELISA | See 1 publications below |
| CoIP | See 1 publications below |
产品信息
HRP-66008 targets DYKDDDDK Tag in WB, CoIP, ELISA applications and shows reactivity with recombinant protein samples.
| 经测试应用 | WB Application Description |
| 文献引用应用 | WB, CoIP, ELISA |
| 经测试反应性 | recombinant protein |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | Flag Tag |
| 别名 | DDDDK tag, DYKDDDDK, DYKDDDDK Tag, HRP conjugated DDDDK tag, HRP-66008, HRP-DDDDK tag |
| 基因名称 | Flag Tag |
| Gene ID (NCBI) | 8 |
| RRID | AB_2883835 |
| 偶联类型 | HRP |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
DYKDDDDK tag, also known as DDK tag, is a hydrophilic tag for recombinant protein technology with the sequence DYKDYKDDDDK. The structure of DYKDDDDK tag has been optimized for compatibility with the proteins it is attached to and unlikely to denature or inactivated them. For its high hydrophilic nature, the DYKDDDDK tag is likely to be located on the surface of a fusion protein, which enables the tag more likely to be accessible to antibodies. This product is generated against 1xDYKDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DYKDDDDK tags, no matter it is/they are located at N-terminal, C-terminal or at internal regions. The binding of this product with DYKDDDDK tagged fusion protein does not depend on calcium ion. The clone of this monoclonal antibody hybridoma is 1E7B4, which is different from any other vendors. This antibody is HRP conjugated DYKDDDDK Tag antibody. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HRP DYKDDDDK Tag antibody HRP-66008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Cancer Exosomal circ_0006896 promotes AML progression via interaction with HDAC1 and restriction of antitumor immunity | ||
Nat Commun A lung-selective delivery of mRNA encoding broadly neutralizing antibody against SARS-CoV-2 infection | ||
Nat Commun FAM222A encodes a protein which accumulates in plaques in Alzheimer's disease. | ||
PLoS Pathog Vibrio splendidus infection promotes circRNA-FGL1-regulated coelomocyte apoptosis via competitive binding to Myc with the deubiquitinase OTUB1 in Apostichopus japonicus | ||
Int Immunopharmacol ZAP facilitates NLRP3 inflammasome activation via promoting the oligomerization of NLRP3 | ||
Liver Int The DEAD-box helicase DDX3x ameliorates non-alcoholic fatty liver disease via mTORC1 signalling pathway. |